Unitika Ltd.

Other

Iatron Laboratories Inc.

Kazuhiko Nagata

Hiroshi Nakajima

Hitoshi Kondo

Ryoichi Tsurutani

Tsutomu Mimura

Yasuhiro Kohama

Kenzo Motosugi

Masao Kageyama

Kazutomo Imahori

Tadao Suzuki

Kosuke Tomita

Kazue Kawahara

Akio Motoyama

Toyohiko Suga

Takanari Shiraishi

Tatsuya Mihara

Hitoshi Kondou

A reagent for calcium ion level determination comprising a phospholipid, phospholipase D, choline oxidase, a surfactant and a divalent metal salt. The reagent can easily be prepared and with which quantitative determination of calcium ion can be made over a broad range of a calcium ion concentration with ease and accuracy in a continuous manner.

Reagent for calcium ion level determination

Reagent for measurement of magnesium ion

A method of preparing an angiotensin converting enzyme inhibitor having the amino acid sequence selected from the group consisting of:

Method of preparing angiotensin converting enzyme inhibitors

A reagent for assaying .gamma.-glutamyltranspeptidase (.gamma.-GTP) which contains an amino acid dehydrogenase and a substrate specific to .gamma.-glutamyltranspeptidase activity. The reagent can be used to assay the .gamma.-GTP activity directly from the continuous course of reaction without using a standard substance with known activity. The procedure required is simple and easy, and the reagent is capable of determining .gamma.-GTP activity with good accuracy and without being affected by interfering substances, making it satisfactory for routine use in clinical laboratories.

Reagents for assay of gamma-glutamyltranspeptidase

An angiotensin converting enzyme inhibitor containing Gly-Lys-Glu-Ile-Ile-Val-Lys-Ala-Glu-Arg (SEQ ID No: 1) Gly-Lys-Met-Val-Lys-Val-Val-Ser-Trp-Tyr (SEQ ID No: 2) or Ala-Tyr-Ile-Ala-Ser-Lys-Gly-Leu (SEQ ID No: 3) as an active component.

Angiotensin converting enzyme inhibitor

A heat-resistant adenylate kinase is described whose activity after an incubation in a buffer solution at about 50.degree. C. for about 15 minutes is at least about 80% of the original activity prior to the incubation. This adenylate kinase can be obtained by cultivating a bacterium belonging to the genus Bacillus and collecting adenylate kinase from the resulting culture broth. This heat-resistant enzyme is very stable against heat and, therefore, after isolation, it can be stored for a long period of time compared with the conventional adenylate kinase.

Adenylate kinase and process for the production thereof

Strain UK 788 (FERM-P No. 5141) that belongs to Bacillus stearothermophilus, the cell of which is longer than about 10 microns and which permits easier release of intracellular components than a type culture Bacillus stearothermophilus, IAM 11001 is disclosed. Also, a process for producing a useful enzyme selected from the group consisting of a heat-resistant polynucleotide phosphorylase, heat-resistant maleate dehydrogenase, heat-resistant glucokinase, heat-resistant glucose-6-phosphate dehydrogenase and heat-resistant pyruvate kinase by culturing such UK 788 and recovering the desired enzyme from the culture is disclosed. Since the cell of the strain UK 788 is easy to settle and its membrane is also easy to break, the useful enzymes mentioned above can be efficiently produced on an industrial scale.

Bacillus stearothermophilus strain UK 788 and process for producing a

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Kyoto, Japan

Kazuhiko Nagata