Ajinomoto Co., Ltd.

University of Minnesota

Kazuhiko Matsui

Yoshihiro Usuda

Hisao Ito

Hiroshi Izui

Mika Moriya

Yoshihiko Hara

Shintaro Iwatani

Osamu Kurahashi

Tsuyoshi Nakamatsu

Shinichi Sugimoto

Eiichiro Kimura

Seiko Hirano

Konosuke Sano

Takuji Ueda

Kiyoshi Miwa

Yoshio Kawahara

Jun Nakamura

Gen Nonaka

Yousuke Nishio

Nobuharu Tsujimoto

Yoshinori Tajima

Tomoko Suzuki

Keita Fukui

Yoko Asakura

Akira Imaizumi

Tsuyoshi Ohsumi

Yuta Nakai

Hiroyuki Kojima

Hisashi Yasueda

Shigeru Nakamori

Yury Ivanovich Kozlov

Natalia Viktorovna Stoynova

Shigeo Suzuki

Hitoshi Enei

Sergey Vasilievich Smirnov

Tatyana Abramovna Yampolskaya

Chie Koseki

Tsuyoshi Osumi

Koichi Ito

Tomohiro Kodera

Veronika Aleksandrovna Kotliarova

Mikiko Suga

Katsuaki Sato

Leonid Romanovich Ptitsyn

Eiji Ono

Yumi Matsuzaki

Richard S Hanson

Natalia Sergeevna Eremina

Stephen Van Dien

Naoki Akiyoshi

Yuichiro Tsuji

Sohei Kanno

Kanae Nakamura

Kazue Kawamura

Seizaburo Shiraga

Masaaki Ishida

Frederick J Schendel

Natalia Nikolaevna Samsonova

Kazuyuki Hayashi

Irina Borisovna Altman

Chieko Osumi

Yuri Nagai

Mahito Terabe

Takashi Tanaka

Masaru Terashita

Shigeru Makamori

Noriko Murayama

Yukiko Ishikawa

Kumiko Fukase

Richard Dillingham

Olga Nikolaevna Mokhova

Stephen c/o Ajinmoto Co Inc Van Dien

An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium.

Method for producing an L-glutamic acid and L-aspartic acid using a recombinant microorganism having enhanced expression of a ybjL protein

An L-amino acid is produced by culturing a microorganism of the family Enterobacteriaceae which has the ability to produce an L-amino acid and which has been modified so as to increase the expression of the evgA gene, the gadE gene, and/or the ydeO gene. These genes encode a transcription factor involved in the EvgAS two-component system regulon. The culture takes place in a medium, and the L-amino acid is collected from the medium or cells.

L-amino acid-producing bacterium and a method for producing an L-amino acid

An L-amino acid is produced by culturing a bacterium of the Enterobacteriaceae family which has an L-amino acid-producing ability in a medium containing fatty acids as the carbon source, particularly fatty acids which have been subjected to emulsification or homogenization, to thereby produce and accumulate the L-amino acid in a culture medium; and collecting the L-amino acid from the culture medium.

Method for producing an L-amino acid

An L-amino acid is produced by culturing a microorganism belonging to the family Enterobacteriaceae having an L-amino acid-producing ability and modified so that glycerol dehydrogenase and dihydroxyacetone kinase activities are increased, in a medium containing glycerol as a carbon source to produce and accumulate an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells.

Method of producing L-amino acid

A method for intracellular metabolic flux analysis comprising (a) culturing cells in a medium not containing any isotope-labeled substrate to a target phase of the metabolic flux analysis, (b) adding an isotope-labeled substrate to the medium, and continuing culture and collecting samples from the medium in time course, (c) measuring isotope distribution in an intracellular metabolite contained in the samples collected in time course, (d) performing a regression analysis for measured data and calculating an isotope distribution ratio in a steady state, and (e) analyzing a metabolic flux of the cultured cells by using the calculated isotope distribution ratio.

Intracellular metabolic flux analysis method using substrate labeled with isotope

A bacterium which belongs to the Enterobacteriaceae family and has an ability to produce an L-amino acid such as L-lysine, L-threonine and L-tryptophan and is modified to enhance glutamic acid decarboxylase activity is cultured in a medium to produce and accumulate the L-amino acid in the medium or cells of the bacterium. Then, the L-amino acid is collected from the medium or the cells.

Method for production of L-amino acid

L-Glutamic acid is produced by culturing in a liquid culture medium a microorganism belonging to the genusorand having an ability to produce L-glutamic acid, which increases in an activity of enzyme catalyzing a reaction for L-glutamic acid biosynthesis or which decreases in or is deficient in an activity of an enzyme catalyzing a reaction branching from a pathway for L-glutamic acid biosynthesis and producing a compound other than L-glutamic acid, and collecting produced L-glutamic acid from the culture medium.

L-glutamic acid-producing bacterium and method for producing L-glutamic acid

A microorganism is cultured in a medium, and is able to produce one or two or more kinds of L-amino acids including L-glutamic acid, L-glutamine, L-proline, L-ornithine, L-citrulline and L-arginine, and is modified to increase &#x3b1;-ketoglutarate synthase activity. The L-amino acids are collected from the medium or the cells.

Microorganism producing an amino acid of the L-glutamic acid family and a method for producing the amino acid

An organic acid is produced by allowing a bacterium which has an ability to produce an organic acid and has been modified so that expression of yidE gene is enhanced, or a product obtained by processing the bacterium, to act on an organic raw material in a reaction mixture containing carbonate ions, bicarbonate ions, or carbon dioxide gas to produce the organic acid, and collecting the organic acid.

Method for producing an organic acid

A bacterium belonging to the genuswhich has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and cause accumulation of L-lysine or L-threonine, and collecting the L-lysine or L-threonine from the medium.

Method for producing L-lysine or L-threonine

A microorganism is provided which can metabolize a carbon source at a specific pH in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source, and which has ability to accumulate L-glutamic acid in an amount exceeding the amount corresponding to the saturation concentration in the liquid medium at the pH. Also provided is a method for producing L-glutamic acid by fermentation, which comprises culturing the microorganism in a liquid medium of which pH is adjusted to a pH at which L-glutamic acid is precipitated, to produce and accumulate L-glutamic acid and precipitate L-glutamic acid in the medium.

Method for producing L-glutamic acid by fermentation accompanied by precipitation

A microorganism is provided which has an ability to produce an L-amino acid such as L-lysine, L-tryptophan, L-phenylalanine, L-valine, L-leucine, L-isoleucine and L-serine, and has been modified to increase the activity of pyruvate synthase or pyruvate:NADPoxidoreductase. This microorganism is cultured in a medium containing ethanol or an aliphatic acid as the carbon source to produce and accumulate the L-amino acid in the medium or cells, and the L-amino acid is collected from the medium or the cells.

Amino acid producing microorganism and a method for producing an amino acid

A metabolic flux affecting substance production using cells is determined by 1) creating a stoichiometric matrix based on formulas of biochemical reactions from a substrate through a desired produced substance, 2) selecting the same number of independent metabolic fluxes from all metabolic fluxes as the degree of freedom of the stoichiometric matrix as free fluxes, 3) creating a sufficient number of random combinations of the free fluxes for a statistical analysis and calculating a metabolic flux distribution from each created combination based on the stoichiometric matrix, 4) obtaining a regression equation including a minimum number of free fluxes that shows a correlation with substance production from the calculated metabolic flux distributions by a multivariate statistical analysis, and 5) determining at least one metabolic flux affecting substance production based on a coefficient in the obtained regression equation.

Method for determining metabolic flux affecting substance production

The present invention provides novel polypeptides and polynucleotides useful as biotechnological tools, specifically identified in a coryneform bacteriumssp.and methods of producing substances in organisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.

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Kawasaki, Japan

Kazuhiko Matsui