Olympus Corporation

Kazuhiko Hosono

Kenji Kawasaki

Masahito Dohi

Daisuke Nishiwaki

Hiroaki Kinoshita

Shingo Tamano

Masahiro Sakakura

A laser scanning microscope includes: an objective that irradiates a specimen with a laser beam; a detection lens that condenses the laser beam that passes through the specimen, the detection lens being arranged so as to face the objective; an optical element that is removably arranged between an image plane on which the detection lens forms an image of the specimen and a first surface that is a lens surface closest to the specimen of the detection lens, the optical element converting the laser beam made incident on the optical element into diffused light or deflecting a portion of the laser beam made incident on the optical element; and a photodetector that detects detection light emitted from the optical element arranged between the image plane and the first surface to the image plane.

Laser scanning microscope, and laser scanning microscope control method

A microscope adapter unit disposed on an optical path of illumination light between a light source unit including a light source and a sample surface includes a first lens group having at least one lens and a second lens group having at least one lens. The first lens group converts the illumination light into roughly parallel luminous fluxes, and makes the illumination light enter the second lens group.

Microscope adapter unit

A fluorescence observation or fluorescence measuring system has at least one of a low-fluorescence objective lens including optical elements made of low-fluorescence glass, satisfying Condition (a) described below, a low-fluorescence immersion substance satisfying Condition (b) described below, and a low-fluorescence cover glass satisfying Condition (c) described below:&#x2266;0.7&#x2003;&#x2003;(a)&#x2266;0.7&#x2003;&#x2003;(b)&#x2266;0.7&#x2003;&#x2003;(c)where B', B&#x2032;, and B&#x2032; are average intensity values of auto-fluorescence from the low-fluorescence objective lens, immersion substance, and cover glass, respectively, and B, B, and Bare average intensity values of auto-fluorescence from a conventional objective lens, immersion substance, and cover glass, respectively, generally used.

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Tokyo, Japan

Kazuhiko Hosono