Sysmex Corporation

The University of Tokyo

Kaya Tai

Hiroyuki Aburatani

Masahiro Kajita

Noriko Oka

Ayako Sakai

Noriaki Yamamoto

Yoshihiko Tashima

Genta Nagae

A determination device for enabling a computer to carry out a process including the steps of: obtaining an analysis result on methylation status of a CpG site located in a promoter region of at least one gene selected from HOXB4 (Homeobox B4) and ZSCAN31 (zinc finger and SCAN domain containing 31) in a DNA sample derived from a subject; and outputting a determination result as information on lung cancer in the subject based on the resulting analysis result is provided. A computer readable medium and a marker for obtaining information on lung cancer are also provided.

Determination device, computer readable medium, and marker for obtaining information on lung cancer

Success or failure of unmethylated cytosine conversion treatment is determined by using a first primer set comprising plural primers that hybridize with a nucleic acid comprising a nucleotide sequence not containing cytosine in the nucleotide sequence of biological DNA that is subject to an unmethylated cytosine conversion treatment and a second primer set comprising plural primers that hybridize with a nucleic acid comprising a nucleotide sequence in which cytosine in a nucleotide sequence containing cytosine and not containing a CpG site is converted into a base other than cytosine, in the nucleotide sequence of biological DNA.

Kit and method for determining whether or not unmethylated cytosine conversion treatment is properly carried out and method for analyzing methylated DNA using the same

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Kobe, Japan

Kaya Tai