Pioneer Hi-Bred International, Inc.

E.i. Dupont De Nemours and Company

Kay Walter

Takashi Yamamoto

Yi Zheng

Naga Kishore Kakani

Ellaine Anne Mariano Fox

Linda A Castle

Steven Gutteridge

Daniel Siehl

Sean M Bertain

Henrik Albert

John Lawrence Andreassi, Ii

This disclosure relates to the field of molecular biology. Provided are novel genes that encode pesticidal proteins. These pesticidal proteins and the nucleic acid sequences that encode them are useful in preparing pesticidal formulations and in the production of transgenic pest-resistant plants. Methods to create or alter pesticidal proteins are provided for altered or enhanced pesticidal activity.

Insecticidal proteins and methods for their use

Compositions and methods comprising polynucleotides and polypeptides having ALS activity and tolerance to at least one ALS inhibitor are provided. In specific embodiments, the sequence has an increased preference for 2-ketobutyrate, when compared to an appropriate control, such as for example, HRA, and/or a preference for 2-ketobutyrate similar to a native ALS. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the ALS inhibitor tolerant sequences. Various methods of employing the ALS inhibitor tolerant sequences are provided. Such methods include methods for producing an ALS inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.

Methods and compositions involving ALS variants with native substrate preference

Growing community of inventors

Sunnyvale, CA, United States of America

Kay Walter