Illumina Corporation

Illumina Cambridge Limited

Illumina Singapore Pte. Ltd.

Kay Klausing

Min-Jui Richard Shen

Helmy A Eltoukhy

Xiaohai Liu

Jonathan Mark Boutell

Robert C Kain

Geoffrey Paul Smith

Wenyi Feng

Xiaolin Wu

Bernard Hirschbein

Sergio Peisajovich

Randall Smith

Thomas J Joseph

Saurabh Nirantar

Misha Golynskiy

Seth McDonald

Carolyn Tregidgo

Hamed Tabatabaei Ghomi

Vincent Peter Smith

John Moore

Kevin Hall

Kevin L Gunderson

Boyan Boyanov

Tarun Kumar Khurana

Bradley Kent Drews

Cyril Delattre

Liangliang Qiang

Sz-Chin Steven Lin

Jennifer Olivia Foley

Lea Pickering

Nicholas Watson

Paul Sangiorgio

Murphy Hitchcock

Thomas Joseph

Roberto Rigatti

Niall Anthony Gormley

Wesley A Cox-Muranami

Wesley A Cox-muranami

Avgousta Ioannou

Mariko Matsuura

Ryan Craig

Kyrie Johnson

Eric Murtfeldt

Alexandra Exner

Epameinondas Fritzilas

Cyril De Lattre

Paul Sangiorgi

Vincent Smith

Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.

Polymerases, compositions, and methods of use

Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain low pre-phasing rates when using ambiently stored polymerases, as well as methods and kits using the same.

A system includes a fluidic device, a flow control valve, a first reagent fluid reservoir fluidly connectable to the fluidic device by the flow control valve, a first fluid buffer reservoir fluidly connectable to the fluidic device by the flow control valve, and a common fluid buffer source fluidly connectable to the fluidic device by the flow control valve. The flow control valve permits flow comprising: (i) flow from the first reagent fluid reservoir to the fluidic device, (ii) flow from the common fluid buffer source to the fluidic device, (iii) flow from the fluidic device to the first fluid buffer reservoir, (iv) flow from the first reagent fluid reservoir to the fluidic device, and (v) flow from the first fluid buffer reservoir to the fluidic device.

System and method for sequestered wash buffer reuse

The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.

Methods and compositions for nucleic acid sequencing

Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.

Compositions, systems, and methods for detecting the presence of polymer subunits using chemiluminescence

System and method for sequestered wash buffered reuse

A method of inhibiting light-induced degradation of nucleic acids includes irradiating a portion of the nucleic acids in the presence of a detection solution comprising a polyphenolic compound. A method of detecting a nucleic acid having a fluorescent tag includes irradiating at least a portion of the nucleic acid with light of a suitable wavelength to induce a fluorescence emission and detecting the fluorescence emission. Optionally, the polyphenolic compound is gallic acid, a lower alkyl ester thereof, or mixtures thereof. A kit includes one or more nucleotides, an enzyme capable of catalyzing incorporation of the nucleotides into a nucleic acid strand and a polyphenolic compound suitable for preparing a detection solution.

Method for nucleotide detection

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San Diego, CA, United States of America

Kay Klausing