The Green Cross Corporation

Katuhiro Uriyu

Yahiro Uemura

Satoshi Funakoshi

Kazuo Takechi

Yutaka Hirao

Tadakazu Suyama

Masayuki Nishida

Tsuyoshi Takahashi

Takashi Goto

Katashi Nakane

Akio Yamane

Masahiro Funayama

A method of producing immunoglobulin preparations for intravenous injection which starts with an immunoblobulin-containing fraction and comprises the treatment steps of:

Method of producing immunoglobulin preparations for intravenous injection

An intravenously injectable solution of chemically unmodified .gamma.-globulin having a complete molecular structure is disclosed, said solution containing sorbitol as a stabilizer and having a low electrical conductivity and a pH of about 5.5.+-.0.2. The injectable solution does not cause an increase of .gamma.-globulin polymer, a rise of anticomplement titer, or impairment of the activities of the .gamma.-globulin, either during preservation or upon administration to a living body.

Gamma-globulin injectable solutions containing sorbitol

A process for heat treating an aqueous solution containing chemically unmodified .gamma.-globulin, wherein said heat treating is carried out in the presence of sorbitol is disclosed. By the heat treatment, impurity viruses can be inactivated without causing impairment of activities of chemically unmodified .gamma.-globulin, increasing polymer contents or increasing an anticomplement titer.

Process for heat treating chemically unmodified gamma-globulin

Virus-contaminated immunoglobulin can be virus-inactivated by heating it in a substantially dry state at a temperature of 30.degree. to 100.degree. C. for a period of time sufficient for inactivating virus with maintaining original activity of the immunoglobulin. The addition of glycine, sodium chloride, sodium acetate, polyethylene glycol, albumin or mannitol enhances the effect and gives good solubility and good state to the solution of the virus-inactivated immunoglobulin.

Process for the virus-inactivation of immunoglobulin

A method for stabilizing plasminogen which comprises adding a physiologically acceptable inorganic salt to a plaminogen-containing aqueous solution to give a final concentration of the salt of 0.002 to 0.4 M.

Method for stabilizing plasminogen

A method of treating hypoproteinemia is disclosed in which an enteric film coated IgA-rich gamma globulin is administered orally to a person suffering therefrom until total serum content and plasma content are restored. The film coating is soluble in neutral or alkaline media by insoluble in acid media.

Employment of enteric coated IgA for hypoproteinemia in intestinal

Highly purified urokinase is obtained in a high yield by adsorbing urokinase from an aqueous crude urokuinase solution including human urine at a pH of about 4.0 to about 7.0 on a water-insoluble polysaccharide containing agarose and agaropectin, for example, agar and Sepharose, and by eluting urokinase with an alkaline solution (pH of about 8 to 11) or concentrated salt solution.

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Sakurai, Japan

Katuhiro Uriyu