National Institute of Advanced Industrial Science and Technology

National Institute of Technology and Evaluation

National Research Institute of Brewing

Tohoku University

Amano Enzyme Inc.

Kanazawa Institute of Technology

Tohoku Techno Arch Co., Ltd.

Katsuya Gomi

Keietsu Abe

Motoaki Sano

Kiyoshi Asai

Masayuki Machida

Naotake Ogasawara

Hideki Nagasaki

Osamu Akita

Akira Hosoyama

Taishin Kin

Satoru Kuhara

Shotaro Yamaguchi

Yasuji Koyama

Katsuhiko Kitamoto

Kotaro Ito

Atsuki Toumoto

Kenichiro Matsushima

Akira Yoshimi

Kensuke Yuuki

Manabu Arioka

Kei Yoshimi

Satoru Hisahara

Misao Sunagawa

Tasuku Nakajima

Ken Miyazawa

Fuka Tabata

Taishin c/o Aist Tokyo Waterfront Kin

Kiyoshi c/o Aist Tokyo Waterfront Asai

Masayuki c/o Tsukuba Central 6 Machida

Motoaki c/o Tsukuba Central 6 Sano

Shotaro c/o Gifu R & D Center Yamaguchi

Hideki c/o Aist Tokyo Waterfront Nagasaki

Fumihiko Hasegawa

M NatInstof AdvIndustScience&Techn Machida

Yohei Yamagata

Hiroshi Room 203 Rest Famil Maeda

Provided is a mutant filamentous fungus, which lacks expression of &#x3b1;-1,3-glucan, and is deficient in at least part of a GAG biosynthetic cluster. Also provided is a method of producing a substance, including the steps of: culturing the filamentous fungus to allow the filamentous fungus to produce a substance; and collecting the resulting substance.

Mutant filamentous fungus and substance production method in which said mutant filamentous fungus is used

An object to be achieved by the present invention is to culture a filamentous fungus at a high density, thereby enabling mass production of a useful substance. The present invention provides a method of producing a substance, including the steps of: culturing a mutant filamentous fungus with no expression of &#x3b1;-1,3-glucan to allow the filamentous fungus to produce a substance; and collecting the resulting substance.

Method for manufacturing useful substance in which high-density cultured strain of filamentous fungi is used

It is intended to provide koji mold-origin phospholipase Aand a DNA encoding it. Namely, phospholipase Acomprising the following protein (a) or (b): (a) a protein having an amino acid sequence represented by SEQ ID NO: 1 or 2; and (b) a protein having an amino acid sequence derived from an amino acid sequence represented by SEQ ID NO: 1 or 2 by a partial modification and serving as phospholipase A.

[object Object]

Lysyl oxidase derived from filamentous fungi and a DNA encoding thereof are provided. Lysyl oxidase including a protein described in of the following (a) or (b): (a) a protein having an amino acid sequence set forth in SEQ ID NO: 2; or (b) a protein having an amino acid sequence obtained by modifying a part of the amino acid sequence set forth in SEQ ID NO: 2, and functioning as lysyl oxidase.

Fungus-origin lysyl oxidases

The purpose of the present invention is to isolate a gene encoding novel exo-, or endo-&#x3b2;--glucanase, to obtain a microorganism having an enhanced expression of said gene and to degrade &#x3b2;--glucan to its low molecular weight form by means of said enzymes. The present invention relates to a gene or DNA encoding novel enzymes having &#x3b2;--glucanase activity (exo-, or endo-&#x3b2;--glucanase), a recombinant expression vector comprising them, a microorganism having the recombinant expression vector, the novel enzymes having &#x3b2;--glucanase activity, and a method for the production of said enzymes.

Genes of cell wall-degrading enzymes derived from aspergillus, and method for the production the enzymes

A completely novel glutaminase is provided: (a) a protein consisting of an amino acid sequence represented by SEQ ID NO: 2, or (b) a protein consisting of an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 2 by deletion, substitution or addition of one or more amino acids, and possessing glutaminase activity. This protein is a novel glutaminase possessing excellent thermostability and the like.

Thermostable glutaminase and thermostable glutaminase gene

Growing community of inventors

Sendai, Japan

Katsuya Gomi