Hiroshima Industrial Promotion Organization

Japan Science and Technology Corporation

Research Development Corporation of Japan

Other

Terumo Kabushiki Kaisha

Phoenixbio Co., Ltd.

Biointegrence Inc.

Japan Science and Technology Agency

Riken Corporation

Hiroshima University

Koken Company, Limited

Toyo Advanced Technologies Co., Ltd.

Research Development Corportion of Japan

Katsutoshi Yoshizato

Chise Mukaidani

Yuichi Mori

Ken Tatebe

Takeo Katakura

Jun Konishi

Mikio Koide

Kaori Oyamada

Takashi Matsuzaki

Mutsumi Inamatsu

Ken-ichi Ohsaki

Toshiaki Takezawa

Miho Kataoka

Teruo Miyata

Michihiro Hide

Susumu Yoshida

R Andrew Cuthbertson

Toshio Taira

Hidekatsu Yoneda

Masanobu Obara

Dan Bach Kristensen

Masaaki Ono

Chise Tateno

Koei Toyoshima

Mikaru Matsunaga

Tatsuhiko Tsunoda

Fuyuki Miya

Hajime Sato

Tomoko Tsutsui

Chihiro Yamasaki

Norio Masumoto

Shingo Ogawa

Masashi Iizuka

Takahiro Adachi

Katsuhiko Shimizu

Masahiro Tomita

Takashi Shimada

Tatsuhiko c/o Riken Tsunoda

Fuyuki c/o Riken Miya

Mibo Kataoka

Disclosed is a nonhuman animal showing the symptoms of human nonalcoholic steatohepatitis which is obtained by transplanting human hepatocytes into an immunodeficient hepatopathic nonhuman animal to produce a chimeric nonhuman animal and then transplanting human hepatocytes that are propagated in the body of the chimeric nonhuman animal into an immunodeficient hepatopathic nonhuman animal of the same species as the immunodeficient hepatopathic nonhuman animal described above, as well as a nonhuman animal showing the symptoms of human fatty liver which is obtained by transplanting human hepatocytes into an immunodeficient hepatopathic nonhuman animal to produce a chimeric nonhuman animal.

Nonalcoholic steatohepatitis model rodent and fatty liver model rodent

A method for culture of hair follicular dermal sheath cells or precursor cells thereof which are potent cellular materials for such as hair regeneration by cell transplantation is provided. That is, by performing culture in an animal cell culture medium supplemented with platelet-derived growth factor AA (PDGF-AA) and fibroblast growth factor 2 (FGF2), hair follicular dermal sheath cells are proliferated while sustaining their function, or hair follicular dermal sheath precursor cells are differentiated into dermal sheath cells and proliferated.

Method for cultivation of hair follicular dermal sheath cells

Human adult hepatocytes are transplanted into an immunodeficient hepatopathy mouse and then human growth hormone is administered to the mouse to thereby elevate twice or more the replacement ratio by the human adult hepatocytes having been transplanted. Further, human growth hormone is administered to an immunodeficient hepatopathy mouse carrying human young hepatocytes transplanted thereinto so as to improve fatty liver of the mouse in which about 70% or more of the hepatocytes have been replaced by the human hepatocytes.

Method of treating mouse carrying human hepatocytes

Transplanting human hepatocytes into a liver of an immunodeficient hepatopathy mouse, and then feeding the mouse transplanted with the human hepatocytes under such a condition as being protected from the attack by human complement produced by the human hepatocytes thereby proliferating the transplanted human hepatocytes in the mouse liver. Further, obtaining human hepatocytes in large scale by repeating the above steps using the proliferated human hepatocytes.

Method of proliferating human hepatocytes and method for obtaining human hepatocytes

The invention provides a method for evaluating the physiological activity of an external stimulus on living cells by means of a surface plasma resonance analyzer, which comprises evaluating the physiological activity of the external stimulus on the cells with an indication of a secondary signal appearing after a primary signal upon the exposure of the living cells to the external stimulus. According to the invention, the level of the physiological activity of an external stimulus on living cells can be evaluated accurately.

Method of evaluating cell activity

The invention provides a monoclonal antibody which specifically recognizes proliferative human hepatocytes, a method for isolating proliferative human hepatocytes by using the antibody, and a method for inducing to differentiate the proliferative human hepatocytes to functional human hepatocytes. Further, the invention provides the proliferative human hepatocytes and functional human hepatocytes obtained by the methods, as well as a cell kit and a hybrid artificial liver comprising those hepatocytes.

Antibody recognizing proliferative human liver cells, proliferative human liver cells and functional human liver cells

The thin film of the invention comprises a hydrate of a vitrified gel containing one or more extracellular matrix components, which can be integrated with a retainer as required. A hydrogel thin film containing one or more extracellular matrix components such as thin-film collagen hydrogel thin film, which is useful for a cell culture substratum and for preventing organ adhesion, can be easily prepared, and is excellent in expediency.

Hydrogel thin film containing extracellular matrix components

A method for identifying phosphoprotein, which comprises: subjecting a sample protein to two-dimensional electrophoresis; dephosphorylating the sample protein with phosphatase; once again performing the two-dimensional electrophoresis under the same conditions; and detecting the spot that migrates to the alkaline side of the isoelectric focusing as the phosphoprotein is provided as a convenient method for identifying phosphoprotein with high accuracy.

Method for identifying phosphoprotein

The present invention provides a biologically active substance secreting hybrid gel, which consists essentially of a biopolymeric gel and cells containing an expression vector with a gene encoding the biologically active substance to produce the substance. According to the present invention, it is possible to develop a gene therapy by skin transplantation allowing stable drug medication for a long time; alleviating pains of patients; and allowing fine adjustment of the dosage and control of genes externally without using retrovirusderived vector that tend to invoke the risk of mutation to wild types as in the conventional prescription.

Biologically active substance-secreting hybrid gel

The present invention provides liver parenchymal cells having a clonal growth ability, which possesses at least one of the cell biological properties such as: presence of peroxysome; being positive to hepatocyte-markers; being partially positive to neoplastic hepatocyte-markers or immature hepatocyte-markers; being positive to antibodies against the surface antigens of ovall cells; and being partially positive to bile duct cell-markers. The present invention also provides a method for obtaining such cells and a method for subculturing such cells. With the liver parenchymal cells above, it will be possible to research in detail the process of development and differentiation, the mechanisms of growth and functional expression of hepatic cells, and to open up a new way to clalification of mechanisms or hepatoma and various other diseases and to development of therapeutic method against these disseases.

Liver parenchymal cells having clonal growth ability, method for

This invention provides a collagen contraction inhibitor, which contains a peptide comprising at least the amino acid sequence of Sequence ID No. 1, its pharmacologically acceptable derivatives, its pharmacologically acceptable salts or a mixture thereof. This peptide specifically inhibits the binding between collagen and fibronectin by the antagonistic action, providing superb effect in the treatment of cheloid and vulnera.

Peptide and collagen contraction inhibitor

This invention provides a method for culturing dermal papilla cells with at least either of the mammalian epidermal cells from the sole or other portions of a mammal and the conditioned medium thereof, in order to permit long stable subculture of dermal papilla cells while keeping the original function thereof intact.

Method for long term subculture of dermal papilla cells

The present invention provides a medium-penetrating culture carrier comprising a plurality of natural or synthetic threads or the woven body thereof, a method for adhering cells onto this carrier to allow them to be proliferated, and a device which is physically connected to the carrier for feeding a medium using this medium-penetrating culture carrier, which are able to culture three-dimensionally animal cells in order that they can effect self-assembly as they do in the living tissue or organ from which they are derived.

Medium-penetrating cell culture carrier, a culturing method and a device

This invention provides a method for preparing hybridomas, which include the steps of: quickly and deeply freezing hair-bulbs of follicle isolated from a human or an animal of species A; immunizing an animal of species B by subcutaneously administering the hair-bulbs after being thawed out to the animal; re-immunizing the animal by intravenously and intraperitoneally administering liquid and solid components of hair-bulbs obtained by centrifugation of minced thawed hair-bulbs to the animal; fusing spleen cells or lymphoid cells isolated from the immunized animal with myeloma cells derived from an animal of species B or C, and cloning fusion cells which produce antibodies capable of specifically binding to the hair follicle of human or animal of species A. The hybridomas prepared by the above methods can produce monoclonal antibodies specifically recognizing hair follicle tissues, and these antibodies enable functional analysis of hair follicle in addition to development of new diagnostic and therapeutic means for abnormal hair development, alopecia, etc.

Method for producing monoclonal antibodies specific to hair follicles

Cell-penetrable medical material made of denatured collagen which is prepared by heating, in the presence of water is disclosed, the collagen having cross-linking structure. Artificial skin prepared by using this medical material as a wound-contacting layer (2) is also disclosed.

Cell-penetrable medical material and artificial skin

Growing community of inventors

Hiroshima, Japan

Katsutoshi Yoshizato