Kao Corporation

Agency of Industrial Science and Technology

Nara Institute of Science and Technology

Katsutoshi Ara

Susumu Ito

Kazuaki Igarashi

Shuji Kawai

Katsuhisa Saeki

Katsuya Ozaki

Keiji Endo

Yuji Hatada

Hiroshi Kusuoku

Katsuhiko Deguchi

Masaki Tosaka

Naotake Ogasawara

Shunichi Akiba

Shenghao Liu

Taeko Sone

Eiichi Hoshino

Yoshinori Nishizawa

Kenzo Koike

Kikuhiko Okamoto

Tohru Kobayashi

Shigeyoshi Tanaka

Kazushi Oshino

Hiroshi Hagihara

Masatoshi Tohata

Akihito Kawahara

Mikio Takaiwa

Yoshiharu Kimura

Hirohiko Ishida

Atsushi Tanaka

Kouji Nakamura

Shigehito Adachi

Shigeo Inoue

Toyoki Hagura

Hiroshi Kakeshita

Hiroshi Kodama

Takuya Morimoto

Sakuya Tanaka

Hajime Mori

Hiromi Okoshi

Takaaki Uemura

Ryosuke Kadoya

Syunichi Akiba

Ryosuke Tochigi Research Institute Kadoya

The present invention provides a modified promoter, an expression vector and a transformant each containing the promoter, and a method for producing a gene product of interest using the transformant. The invention provides a modified promoter, including a nucleotide sequence of a promoter derived from bacterium belonging to the genusin which at least one nucleotide sequence selected from the following has been modified: a nucleotide sequence represented by SEQ ID NO: 1; a nucleotide sequence equivalent to the nucleotide sequence represented by SEQ ID NO: 1, except that one or a plurality of bases therein are substituted, deleted, added or inserted; and a nucleotide sequence having a sequence identity of 70% or more with respect to the nucleotide sequence represented by SEQ ID NO: 1.

Modified promoter

A method of modifying a target region in a host DNA using a donor DNA: wherein the donor DNA having regions homologous to a 5'-side region outside of the target region in the host DNA, a 3&#x2032;-side region outside of the target region in the host DNA and a first homologous recombination region inside of the target region in the host DNA, respectively, in this order, and further having a first selectable marker gene, an expression-inducing promoter and a second selectable marker gene expressed under the control of the expression-inducing promoter between the region homologous to the 3&#x2032;-side region and the region homologous to the first homologous recombination region; which method has the steps of: a first step of performing homologous recombination between the donor DNA and the host DNA at the regions of the 5&#x2032;-side region and the first homologous recombination region, to conduct selection of a host integrated with the donor DNA based on expression of the first selectable marker gene; and a second step of performing homologous recombination, within the host DNA integrated with the donor DNA by the first step, between two regions of the 3&#x2032;-side region derived from the host DNA and the 3&#x2032;-side region derived from the donor DNA, to conduct selection of a host whose target region is modified based on expression of the second selectable marker gene under an expression-inducing condition for the expression-inducing promoter; and a selectable marker cassette for use in the method.

Method of modifying target region in host DNA and selectable marker cassette

Deodorant agents, which containorBark or its extract as an active ingredient and which are high in safety and can radically inhibit the emission of human body malodors typified by sweat odor, especially, axillary odor.

Deodorant agent

The object of the invention is to provide a microorganism which enhances productivity of cellulase and is useful for industrial production of cellulase, and to provide a method for producing cellulase by use of the microorganism. The present invention provides a recombinant microorganism produced by transferring a gene encoding cellulase to a parental microorganism which has been genetically modified so as to overexpress asecY gene or a gene corresponding thereto.

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A recombinant microorganism having improved productivity for a protein or a polypeptide, and a method for producing a protein or a polypeptide using the recombinant microorganism, are provided. A recombinant microorganism obtained by transfecting a gene for encoding a desired protein or polypeptide into a microorganism strain which is obtained by genetically constructing to overexpress secY gene ofor a gene corresponding to the secY gene, and deleting or inactivating one or more genes selected from sporulation-associated genes and genes corresponding to the sporulation-associated genes from the genome.

A recombinant microorganism is provided that has increased productivity of a protein or polypeptide of interest. A method for producing a protein of interest using the microorganism is also provided. The microorganism is prepared by inserting, in its genome, a transcription initiation regulatory region or both a transcription initiation regulatory region and a ribosome-binding site, upstream of aprsA gene and by deleting or inactivating one or more of the abrB gene, dltA gene, dltB gene, dltC gene, dltD gene, dltE gene, or a gene (genes) corresponding thereto.

Recombinant microorganism

To provide a microorganism with enhanced secretory production of a protein or polypeptide and a method of producing the protein or polypeptide using the microorganism. A modified microorganism that has been genetically modified to delete 60 to 80 carboxyl-terminal amino acids of SecA.

Modified microorganism

Novelmutant strains having good productivity of various enzymes are provided through extensive analysis of strains that are derived fromvia gene disruption. Themutant strains according to the present invention have genomic structures prepared by deletion of regions listed in the columns for deficient regions. Each of thesemutant strains exerts significantly improved secretory productivity of a protein when a gene encoding such a secretory target protein is introduced so that it can be expressed, compared with a case in which the same gene is introduced into a wild-type strain.

The invention contemplates drugs useful for maintenance or promotion at sites of regenerating nerves and for prevention or treatment of pathological conditions caused by denaturation or cell damage in the nervous system. Thus, the invention provides an apolipoprotein D degradation inhibitor containing lauric acid or oleic acid as an active ingredient.

Apolipoprotein D degradation inhibitor

The present invention is directed to a liquefying alkaline alpha-amylase, and a DNA encoding for the same and functional fragments thereof.

Gene encoding alkaline liquefying &#x3b1;-amylase

The present invention provides: a nucleotide sequence encoding alkaline pullulanase and exhibiting both alkaline &agr;-amylase and alkaline pullulanase activity; a nucleotide sequence encoding alkaline &agr;-amylase possessing an amino acid sequence described in SEQ ID NO:3; a nucleotide sequence encoding alkaline pullulanase possessing an amino acid sequence described in SEQ ID NO:4; recombinant DNAs containing these nucleotide sequences, and transformed microorganisms harboring the recombinant DNAs. The technique of the present invention enables mass production of alkaline pullulanase exhibiting both alkaline &agr;-amylase and alkaline pullulanase activity.

Gene for enzyme having both alkaline pullulanase and alkaline alpha-amylase activities

Gene encoding alkaline liquifying alpha-amylase

DNA fragment containing gene for alkaline pullulanase

Citral acetal

The present invention provides a DNA fragment encoding alkaline pullulanase exhibiting alkaline &agr;-amylase activity, alkaline &agr;-amylase possessing both an amino acid sequence described in SEQ ID NO:3 and a DNA fragment encoding the amylase, alkaline pullulanase possessing both an amino acid sequence described in SEQ ID NO:4 and a DNA fragment encoding the pullulanase, recombinant DNAs containing these DNA fragments, and transformed microorganisms harboring the recombinant DNAs. The technique of the present invention enables mass production of alkaline pullulanase exhibiting alkaline &agr;-amylase activity.

Gene for enzyme having both alkaline pullulanase and alkaline &agr;-amylase activities

Growing community of inventors

Haga-gun, Japan

Katsutoshi Ara