Toray Industries, Inc.

National Institute of Advanced Industrial Science and Technology

Riken Corporation

Chiba University

Katsushige Yamada

Hiroyuki Kurihara

Masateru Ito

Kenji Kawamura

Atsushi Minamino

Hideki Sawai

Kenji Sawai

Shingo Hiramatsu

Kyohei Isobe

Satoshi Sakami

Daijiro Tsukamoto

Shigeyuki Funada

Chiaki Yamada

Takashi Mimitsuka

Shin-ichi Minegishi

Jumpei Kishimoto

Izumi Morita

Junpei Kishimoto

Fumiyoshi Okano

Yusuke Kagawa

Koji Kobayashi

Masahiro Satoh

Kazuhiko Ishikawa

Masato Akahira

Yuka Asahi

Yuki Yamamoto

Koji Yamauchi

Yohito Ito

Shinichi Minegishi

Masashi Higasa

Kyousuke Yotsumoto

Hiroko Ishizuka

Haruka Saito

Izumi Nakagawa

Takuya Kasahara

Masayuki Hanakawa

Takahiro Arai

Daisuke Yamamoto

Kohei Yamashita

Isao Kawakami

Tatsuya Nagano

Tetsu Yonehara

Hiromi Tsutsui

Makoto Shirai

Ken Morita

Yumiko Mishima

Yasunobu Wada

Yuji Kado

Shinichi Yamane

Eri Shimizu

Tomiya Uchino

Miyuki Horiguchi

Shiomi Watanabe

Shin-Ichi Minegishi

Mark Joseph Burk

Tetsuya Yamada

Masatoshi Watanabe

Sadanori Kumazawa

Kentaro Ishii

Michael Japs

Masahiro Niwa

Masahiro Henmi

Warren Clark

Akira Yanai

Takashi Tanaka

Koya Kato

Satoshi Sato

Hideshi Yanase

Takanori Nishida

Kazumi Suda

Shigeharu Moriya

Nanami Sasaki

Moriya Ohkuma

Masahiro Watanabe

Masahiro Yuki

Masae Takeuchi

Kyungsu Na

Takeshi Tsukada

Masato Otagiri

Yuka Maeno

Junya Yamagishi

Toshiki Tamura

Ito Masateru

Naomi Hara

Natsuko Murakami

Sho Shindo

Masataka Makino

Hailing Duan

The present invention relates to a method of producing ε-caprolactam, the method including the following steps (A) and (B): (A) a step of reacting 5-cyanovaleramide with hydrogen in an aqueous solvent in a presence of a hydrogenation catalyst to obtain a 5-cyanovaleramide hydrogenation reaction mixture; (B) a step of heating the 5-cyanovaleramide hydrogenation reaction mixture at a temperature of 180° C. or higher and 300° C. or lower in an aqueous solvent to obtain ε-caprolactam.

Method for producing ε-caprolactam

A mutant strain of, the mutant strain having a mutation that eliminates or reduces a function of a polypeptide consisting of the amino acid sequence represented by any of SEQ ID Nos: 4 to 6. A method produces a cellulast, the method including a step of cultivating the mutant strain of, the mutant strain having a mutation that eliminates or reduces a function of a polypeptide consisting of the amino acid sequence represented by any of SEQ ID Nos: 4 to 6.

Mutant strain ofand method of producing protein using same

Disclosed is a genetically modified microorganism into which a nucleic acid encoding an enzyme that catalyzes a reaction to reduce 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA is introduced. In the genetically modified microorganism, a nucleic acid encoding any one of the polypeptides described in (a) to (c) below is introduced or expression of the polypeptide is enhanced: (a) a polypeptide composed of an amino acid sequence represented by any one of SEQ ID NOs: 1 to 6 and 213, (b) a polypeptide composed of the same amino acid sequence as any one of the amino acid sequences, except that one or several amino acids are substituted, deleted, inserted, and/or added, and having enzymatic activity that catalyzes a reaction to reduce 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA, and (c) a polypeptide composed of an amino acid sequence with a sequence identity of not less than 70% to any one of the amino acid sequences and having activity to reduce 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA.

Gene-modified microorganism for producing 3-hydroxyadipic acid, alpha-hydromuconic acid, and/or adipic acid, and production method for said chemical products

An aquatic organism growth promotor, containing, as an effective ingredient, a low molecular weight lignin having a molecular weight peak in a molecular weight range of 4,000 to 9,500 and/or a high molecular weight lignin having a molecular weight peak in a molecular weight range of 10,000 to 40,000, wherein the molecular weight peak is measured at a wavelength of 254 nm by GPC molecular weight analysis using an UV detector.

Aquatic organism growth promotor

A mutant of afilamentous fungus has a mutation that results in lost or lowered function of a beta-adaptin large subunit, or a mutation in an amino acid sequence that forms the beta-adaptin large subunit. A method produces a protein while maintaining a low viscosity in a culture solution during culturing by using the mutant.

Mutant offilamentous fungus and method of producing protein

A method relates to producing 3-hydroxyadipic acid-3,6-lactone, the method including the following steps (A) and (B): (A) a step of adding an acid to a 3-hydroxyadipic acid-containing aqueous solution to obtain a 3-hydroxyadipic acid-3,6-lactone-containing aqueous solution; and (B) a step of obtaining a 3-hydroxyadipic acid-3,6-lactone extract by bringing the 3-hydroxyadipic acid-3,6-lactone-containing aqueous solution obtained in step (A) into contact with an extraction solvent that is phase-separated from the solution.

Method of producing 3-hydroxyadipic acid-3,6-lactone

A method of producing 3-oxoadipic acid from an aliphatic compound easily utilizable by a microorganism, such as a saccharide, by utilization of a metabolic pathway of the microorganism is disclosed. The method of producing 3-oxoadipic acid includes the step of culturing at least one type of microorganism having a capacity to produce 3-oxoadipic acid, selected from the group consisting of, for example, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, and microorganisms belonging to the genus

Method for producing 3-Oxoadipic acid

A mutant strain ofhas a mutation that eliminates or reduces a function of a polypeptide consisting of the amino acid sequence represented by SEQ ID NO: 2. A method produces a protein, the method including a step of cultivating the mutant strain.

mutant strain, and method of producing protein

A mutant strain of a filamentous fungus of the genushaving a reduced function of a polypeptide consisting of the amino acid sequence represented by SEQ ID NO: 2; and a method of producing a sugar from a cellulose-containing biomass, the method including: step a of producing a cellulase by cultivating amutant strain having a reduced function of a polypeptide consisting of the amino acid sequence represented by SEQ ID NO: 2, and step b of saccharifying the biomass by using the cellulase obtained in the step a.

Trichoderma filamentous fungus mutant strain and method of producing protein

Lactic acid is obtained by a method including (A) a step of continuous fermentation wherein a fermentation culture medium of a microorganism having an ability of lactic acid fermentation is filtered through a porous membrane having an average pore size of not less than 0.01 &#x3bc;m and less than 1 &#x3bc;m with a transmembrane pressure difference within the range of 0.1 to 20 kPa, and the permeate is collected, while retaining the non-permeated liquid in or returning the non-permeated liquid to the culture, and adding a fermentation feedstock to the culture; (B) a step of filtering the permeate obtained in Step (A) through a nanofiltration membrane; and (C) a step of distilling the permeate obtained in Step (B) under a pressure of not less than 1 Pa and not more than atmospheric pressure, at 25&#xb0; C. to 200&#xb0; C. to recover lactic acid.

Method for producing lactic acid and method for producing polylactic acid

Amutant strain has a function of a polypeptide consisting of the amino acid sequence represented by SEQ ID NO: 2 is reduced. A method of producing a protein includes a step of cultivating the-mutant strain, and a method of producing a cellulase includes a step of cultivating themutant strain.

Trichoderma reesei mutant and protein production method

A method produces a protein using a filamentous fungus, in which decrease in dissolved oxygen saturation during culture of the filamentous fungus can be suppressed even when the culture is scaled up. The method of producing a protein includes culturing a fungus belonging to the genuswhose BXL1 gene was disrupted, using a biomass containing cellulose and xylan as an inducer. The use of the BXL1 gene-disrupted fungus belonging to the genusenables suppression of the decrease in dissolved oxygen saturation even when xylose and cellulose are used as inducers.

Method of producing protein

A filtration device includes a continuous first unit including a first membrane that separates a liquid into first permeated and non-permeated liquids, a first adjuster that adjusts a flow rate of the first permeated liquid to be substantially constant, and a first liquid scale that detects a liquid amount, a second unit including a second membrane that separates another liquid into second permeated and non-permeated liquids, a second adjuster that adjusts a flow rate of the second permeated liquid to be substantially constant, and a second liquid scale that detects another liquid amount, a first controller that controls the liquid amount in the first storage tank based on measurement values from continuous two first units or from the continuous first and second units, and a second controller that controls the another liquid amount based on a measurement value from the second unit.

Filtration device

A method produces xylo-oligosaccharides from a biomass containing xylan and cellulose, which method is convenient and has a high yield of xylo-oligosaccharides because of inhibition of degradation of xylo-oligosaccharides into xylose. In the method of producing xylo-oligosaccharides, a biomass containing xylan and cellulose is hydrolyzed with a cellulase composition having at least activities of xylanase, cellobiohydrolase and &#x3b2;-glucosidase and substantially free of &#x3b2;-xylosidase activity during hydrolysis.

Method of producing xylo-oligosaccharide

A method of producing cellulase includes steps (1) to (3): (1) subjecting an aqueous solution of cellulase derived from filamentous fungi to filtration through an ultrafiltration membrane with a molecular weight cut off of 100,000 to 200,000 to obtain a filtrate and concurrently obtain a concentrated enzyme liquid as a retentate; (2) further subjecting the filtrate obtained in step (1) to filtration through a second ultrafiltration membrane with a molecular weight cut off of 5,000 to 50,000 to obtain a second concentrated enzyme liquid as a retentate; and (3) mixing the concentrated enzyme liquid obtained in steps (1) and (2) to obtain cellulase derived from filamentous fungi.

Method for producing cellulase and apparatus for said method

Growing community of inventors

Kamakura, Japan

Katsushige Yamada