University of Michigan

Katsuo Kurabayashi

Xudong Fan

Hongbo Zhu

Shuichi Takayama

Dongeun Huh

Steven J Skerlos

Edward T Zellers

Xiaogan Liang

Yi-Chung Tung

James B Grotberg

Menglian Zhou

Jiwon Lee

Brian D Jensen

Kensall D Wise

Thomas D Wang

Kenn Oldham

Bruce P Block

Young Geun Park

Arlyne Simon

Byunghoon Ryu

Zhen Qiu

Edgar Meyhöfer

Chao Yung Fan

Dawen Cai

Pengyu Chen

Robert Nidetz

Meng-Ting Chung

Maxwell Wei-Hao Li

Timothy T Cornell

Jung Hwan Seo

Thomas P Shanley

Sun Kyu Kim

Michael Farina

Daniel Nunez

Meng Ting Chung

Nien-Tsu Huang

Jinyan She

Yujing Song

Sung Jin Kim

Walker M McHugh

Chih-Tin Lin

Amaya Frost

A gas chromatography device for peak focusing of one or more target analytes is provided that include a chromatographic column with an inlet and an outlet. A stationary phase is deposited inside the chromatographic column and has a positive thickness gradient. The stationary phase extends from the inlet to the outlet and has a first thickness at the inlet of the chromatographic column and a second thickness at the outlet of the chromatographic column. The second thickness is at least about 10% greater than the first thickness. Methods of peak focusing in a gas chromatography device, method of verifying peak focusing in a gas chromatography device and creating a gas chromatography device having a chromatographic column with a positive thickness gradient are also provided.

Chromatographic column having stationary phase thickness gradient

An integrated microfluidic photoionization detector (PID) is provided including a microfluidic ionization chamber a microfluidic ultraviolet radiation chamber that is configured to generate ultraviolet photons. An ultrathin transmissive window is disposed between the microfluidic ionization chamber and the microfluidic ultraviolet radiation chamber that permits the ultraviolet photons to pass from the microfluidic ultraviolet radiation chamber into the microfluidic ionization chamber. Detection systems for one or more VOC analytes are also provided that include a gas chromatography (GC) unit including at least one gas chromatography column and an integrated microfluidic photoionization detector (PID) disposed downstream of the gas chromatography (GC) unit.

Integrated micro-photoionization detector with an ultrathin ultraviolet transmission window

Provided herein are systems, methods, and articles of manufacture for collecting and merging two different size droplets using a substrate comprising a plurality of trapping sites. In certain embodiments, provided herein are systems composed of a plurality of larger droplets and smaller droplets and a substrate comprising a plurality of trapping sites where each trapping site is configured to trap only one of the larger droplets and only one of the smaller droplets when the larger droplet is already present at the trapping site. In particular embodiments, the larger and/or smaller droplets are sorted prior to being contacted with the substrate to ensure they contain the desired component (e.g., cell or barcoded bead). In other embodiments, each trapping site is composed of one or multiple fluidically linked capture wells. In some embodiments, collected larger and smaller droplets are merged (e.g., via a demulsifier or electricity).

Multi-droplet capture

Provided herein are systems and methods for performing assays. In particular, provided herein are systems and methods for performing sensitive and rapid immunoassays.

Plasmo photoelectronic immunosensor

Provided herein are systems and methods for assays. In particular, provided herein are systems and methods for performing high throughput immunoassays. Embodiments of the present disclosure provide multiplex capable LSPR immunoassays that meet a need for rapid (e.g., near real time), accurate immunoassays (e.g. for use in beside diagnostics). The LSPR assays are as accurate as existing ELISA assays but provide the advantage of increased speed and multiplex capability. In addition, the LSPR immunoassays are able to analyze small volumes of complex patient samples (e.g., serum).

Systems and methods for performing immunoassays

The present disclosure provides a method for conducting comprehensive chromatography analysis. Broadly, the method comprises separating a sample in a first chromatographic column to generate a primary stream, which is directed toward a non-modulator switching system comprising at least one micro-switch and at least one valve. The non-modulator switching system is continuously operated to: (a) selectively direct a portion of the primary stream to one of a plurality of thermal injectors and accumulating the portion of the primary stream for a predetermined amount of time; (b) inject the portion of the primary stream into one of a plurality of secondary chromatographic columns; (c) detect one or more analytes in a secondary stream exiting the secondary chromatographic column; and repeat (a)-(c) to selectively direct other portions of the primary stream to other thermal injectors and secondary chromatographic columns until all of the analytes in the sample are detected.

Multi-channel multi-dimensional comprehensive gas chromatography

A discharge ionization current detector for ionizing and detecting a sample component where the sample component is ionized by UV light is provided. The detector includes a UV light source, and a microfluidic channel having a first surface including a conducting or semiconducting material and an opposing second surface including a conducting or semiconducting material, one of the first surface and the second surface being a collection electrode and the other of the first surface and the second surface being a bias electrode, the microfluidic channel being configured to receive a sample component. The microfluidic channel is in fluid communication with the UV light source such that, when activated, UV light from the UV light source can enter the microfluidic channel and ionize the sample component, which releases an electron, and the ionized sample component and electron are detected by the collection and bias electrodes.

Miniaturized discharge photo-ionization detector

A rapid flow-through, highly sensitive microfluidic photoionization detector (PID) which is micro-fabricated directly onto a substrate, such as a conductive silicon wafer, is provided. The microfluidic PID has an ionization chamber volume of less than 9 &#x3bc;L. The microfluidic PID may have a flow through design with a microfluidic channel defines a serpentine pattern on the substrate. The flow through design of the microfluidic PID results in negligible dead volume, thus allowing a shortened response time over existing commercially available designs. Such microfluidic PIDs are particularly useful with gas chromatography (GC), including microGC and multi-dimensional microGC systems. Methods for calibrating PIDs are also provided.

Microfluidic photoionization detector

An optical device is described that may be used as a microscope system for real-time, three-dimensional optical imaging. The device includes a miniature, fiber optic, intra-vital probe microscope that uses a dual-axes confocal architecture to allow for vertical scanning perpendicular to a surface of the sample (e.g., a tissue surface). The optical device can use off-axis illumination and collection of light to achieve sub-cellular resolution with deep tissue penetration. The optical device may be used as part of an integrated molecular imaging strategy using fluorescence-labeled peptides to detect cell surface targets that are up-regulated by the epithelium and/or endothelium of colon and breast tumors in small animal models of cancer.

Targeted dual-axes confocal imaging apparatus with vertical scanning capabilities

Atomically layered transition metal dichalcogenides (TMDCs) exhibit a significant potential to enable low-cost transistor biosensors that permit single-molecule-level quantification of biomolecules. Two different principles for operating such biosensors are presented. In one arrangement, antibody receptors are functionalized on an insulating layer deposited onto the channel of the transistor. The charge introduced through antigen-antibody binding is capacitively coupled with the channel and shifts the threshold voltage without significantly changing the transconductance. In another arrangement, antibodies are functionalized directly on the channel of the transistor. Antigen-antibody binding events mainly modulate the ON-state transconductance, which is attributed to the disordered potential formed in channel material.

Two-dimensional material-based field-effect transistor sensors

A passive and reusable preconcentrator/injector device for measuring gas-phase analytes and methods of use. The device includes an upper plate defining an array of micro-scale diffusion channels and a lower plate secured to the upper plate. The lower plate defines a cavity for a reusable collection material in fluid communication with the micro-scale diffusion channels. An integral heating unit is provided adjacent the lower plate and configured for heating the cavity. A loading port may be included for introducing the reusable collection material into the cavity. An inlet port and an outlet port are provided, both in fluid communication with the cavity. The device may include a fluidic manifold system comprising a plurality of conduits disposed between the adsorbent cavity and the outlet port.

Micro-scale passive vapor preconcentrator/injector

The present invention relates to solution microarrays. In particular, the present invention relates to an aqueous 2-phase system for solution microarrays and uses thereof. The present invention further relates to systems and methods for performing assays within the solution microarrays (e.g., diagnostic assays).

Solution microarrays and uses thereof

A two-dimensional gas chromatography system having first and second separation columns, a thermal modulator disposed between the first and second columns and having a plurality of stage channels, and a micro heater monolithically integrated in the thermal modulator. The thermal modulator is operable to trap and focus gas/vapor species within the plurality of stage channels upon cooling and release the gas/vapor species to the second column upon heating by the micro heater.

Microfabricated thermal modulator for comprehensive 2D gas chromatography

A protein patterning electrode device consisting of capacitor microelectrode arrays coated with a protein non-adherent layer is provided. Operation of the electrode is based on a phenomenon called 'electrowetting,' where surface wettability can dynamically be controlled by varying the voltage across the device electrodes. When an electric field is applied across the electrode layers, the surface accumulates charge and becomes hydrophilic, binding the proteins to the surface via ionic bonding. Electrically controlling the amount of the surface charge permits controlled protein surface affinity. The device provides a means for reconfigurable protein patterning.

Reconfigurable protein patterning using electrowetting microelectrodes

Gas focusing flow cytometers are fabricatable employing simple and inexpensive manufacturing techniques. When such cytometers or conventional cytometers are combined with fiber optical light paths and laser diode and semiconductor photodetectors, light weight and handheld, optionally disposable devices which maintain high performance are possible.

Growing community of inventors

Ann Arbor, MI, United States of America

Katsuo Kurabayashi