Nishikawa Rubber Co., Ltd.

Daio Paper Corporation

Katsunori Nakashima

Isao Ohiso

Junya Okawa

Keigo Matsumoto

Takaaki Imai

Syoichi Nakamoto

A surface of a rubber sponge materialsuch as a vehicle weather strip is covered with a resin coatcontaining dispersed cellulose fibers

Sound-insulating sponge material, vehicle sealing material, and weather strip

To provide a method for selectively amplifying a target nucleic acid and a method for detecting a nucleic acid by the method, which are useful as a method for synthesizing the nucleic acid. A method for amplifying a nucleic acid sequence (EVA (Endonuclease V-dependent Amplification) method) which selectively amplifies a target nucleic acid in a sample, by the use of an oligonucleotide primer containing a base which can be recognized by an endonuclease V, the endonuclease V and a DNA polymerase having a strand displacement activity, and a method for detecting a nucleic acid by the method.

Method for amplification of nucleotide sequence

A novel mutant endonuclease V not exhibiting a nonspecific nucleic acid cleavage activity but showing a specific activity. The present invention relates to a mutant endonuclease V not exhibiting a nonspecific nucleic acid cleavage activity but having a specific activity, to a gene coding for the endonuclease V, to a recombinant DNA containing the gene, to a transformant or transductant containing the recombinant DNA, and to a method for producing the endonuclease V. The present invention also relates to a method of using the endonuclease V for nucleic acid cleavage, and to a reagent kit for detection or modification of a nucleic acid or gene that contains the endonuclease V.

Growing community of inventors

Hiroshima, Japan

Katsunori Nakashima