Nec Solution Innovators, Ltd.

Gunma University

Mosaic Biosciences, Inc.

Katsunori Horii

Iwao Waga

Jou Akitomi

Naoto Kaneko

Hirotaka Minagawa

Masayasu Kuwahara

Shintarou Katou

Ikuo Shiratori

Yoshihito Yoshida

Makio Furuichi

Hiromi Takenaka

Mineko Yamaguchi

Hirotaka Yagi

Kensaku Nishikata

Martin Stanton

Tomoko Fujita

Akihisa Shimizu

Shintaro Kato

Tomoko Shiratori

Andrew Dalby

Hiroshi Mishima

Tomoko Murata

The present invention provides a novel nucleoside derivative or a salt thereof, a polynucleotide synthesis reagent, a method for producing a polynucleotide, a polynucleotide, and a method for producing a binding nucleic acid molecule. The nucleoside derivative or a salt thereof of the present invention is represented by the following chemical formula (1).  In the chemical formula (1), Su is an atomic group having a sugar skeleton at a nucleoside residue or an atomic group having a sugar phosphate skeleton at a nucleotide residue, and may or may not have a protecting group, Land Lare each independently a straight-chain or branched, saturated or unsaturated hydrocarbon group having 2 to 10 carbon atoms, Xis an imino group (&#x2014;NR&#x2014;), an ether group (&#x2014;O&#x2014;), or a thioether group (&#x2014;S&#x2014;), and Ris a hydrogen atom or a straight-chain or branched, saturated or unsaturated hydrocarbon group having 2 to 10 carbon atoms.

Nucleoside derivative or salt thereof, polynucleotide synthesis reagent, method for producing polynucleotide, polynucleotide, and method for producing binding nucleic acid molecule

The present invention provides a method for producing a nucleic acid molecule that can obtain a nucleic acid molecule that binds to a target and does not inhibit a function of the target. The production method for a nucleic acid molecule of the present invention is a method for producing a nucleic acid molecule that binds to a first biological molecule and does not inhibit a function of the first biological molecule, the method including the steps of: (A) bringing a candidate nucleic acid molecule into contact with the first biological molecule to select a nucleic acid molecule that has bound to the first biological molecule as a first selected nucleic acid molecule; and (B) selecting the first selected nucleic acid molecule as an intended nucleic acid molecule.

Method for producing nucleic acid molecule, biomaterial, and method for producing biomaterial

The present invention provides a novel molecule that can be used for detection of sIgA. The sIgA-binding nucleic acid molecule of the present invention is characterized in that it binds to secretory immunoglobulin A (sIgA) with a dissociation constant of 37.7 nM or less, and preferably includes a polynucleotide consisting of any of base sequences of SEQ ID NOs: 1 to 12 or a partial sequence thereof, for example. According to the sIgA-binding nucleic acid molecule of the present invention, it is possible to detect sIgA in saliva.

Secretory immunoglobulin a (sIgA)-binding nucleic acid molecule, sIgA analysis sensor, and sIgA analysis method

The present invention provides a novel nucleic acid molecule that can be used for detection of a wheat allergen. The wheat allergen-binding nucleic acid according to the present invention is characterized in that it binds to a wheat allergen with a dissociation constant of 20 nM or less, and preferably includes a polynucleotide consisting of either of base sequences of SEQ ID NOs: 1 and 2, for example.

Wheat allergen-binding nucleic acid molecule and use thereof

The present invention provides a novel fluorescent protein. A novel protein includes the following protein (F1):

Protein, novel gene, expression vector, transformant, method for producing transformant, and method for screening for novel fluorescent protein

The present invention provides a novel nucleoside derivative or a salt thereof, a polynucleotide synthesis reagent, a method for producing a polynucleotide, a polynucleotide, and a method for producing a binding nucleic acid molecule. The nucleoside derivative or a salt thereof of the present invention is represented by the following chemical formula (1): where in the chemical formula (1), Su is an atomic group having a sugar skeleton at a nucleoside residue or an atomic group having a sugar phosphate skeleton at a nucleotide residue, and may or may not have a protecting group, Land Lare each independently a straight-chain or branched, saturated or unsaturated hydrocarbon group having 2 to 10 carbon atoms, Xand Xare each independently an imino group (&#x2014;NR&#x2014;), an ether group (&#x2014;O&#x2014;), or a thioether group (&#x2014;S&#x2014;), and the Ris a hydrogen atom or a straight-chain or branched, saturated or unsaturated hydrocarbon group having 2 to 10 carbon atoms.

The present invention provides a novel nucleic acid molecule that can be used for detection of &#x3b1;-amylase. The &#x3b1;-amylase-binding nucleic acid molecule of the present invention is characterized in that it binds to &#x3b1;-amylase with a dissociation constant of 17 nM or less, and preferably includes a polynucleotide consisting of any of base sequences of SEQ ID NOs: 1 to 22, for example. According to the nucleic acid molecule of the present invention, it is possible to detect &#x3b1;-amylase in saliva.

&#x3b1;-amylase-binding nucleic acid molecule and use thereof

The present invention provides a novel method that can analyze a target easily utilizing binding nucleic acid molecules and an analysis kit for use in the method. The analysis method of the present invention includes: a complex formation step of causing a binding nucleic acid molecule that binds to the target and a sample to come into contact with each other to form a complex of the binding nucleic acid molecule and the target in the sample; a nuclease treatment step of releasing a nucleic acid monomer from at least one of a complex fraction and a non-complex fraction by a nuclease treatment; an enzyme treatment step of reacting the released nucleic acid monomer with an enzyme for which the nucleic acid monomer is a substrate; a detection step of detecting the enzyme reaction; and an analysis step of analyzing the target that has formed the complex from the result of detecting the enzyme reaction.

Method and kit for analyzing target

The present invention provides a novel technique by which the redox activity of a nucleic acid molecule can be evaluated. An evaluation method of the present invention includes: a detection step of electrochemically detecting a redox reaction to a substrate, the redox reaction being catalyzed by a nucleic acid molecule to be evaluated, using a device that electrochemically detects a redox reaction; and an evaluation step of evaluating redox activity of the nucleic acid molecule from a result of the detection of the redox reaction. As the device, a device in which a base provided with a detection portion is included, the detection portion includes an electrode system, and the nucleic acid molecule to be evaluated is arranged on the base is used. In the present invention, it is preferred that a plurality of kinds of nucleic acid molecule to be evaluated is arranged on the base, and the plurality of kinds of nucleic acid molecules to be evaluated is evaluated by a single device.

Method for evaluating redox activity of nucleic acid molecule and nucleic acid molecule having redox activity

The present invention is intended to provide a novel fluorescence sensor for target analysis, a kit for target analysis, and a target analysis method using the same. The fluorescence sensor for target analysis according to the present invention includes a nucleic acid molecule that includes a G-quartet-forming nucleic acid region (D) that forms a G-quartet and a binding nucleic acid region (A) that binds to a target. In the absence of a target, formation of a G-quartet in the G-quartet-forming nucleic acid region (D) is inhibited. In the presence of a target, the target comes into contact with the binding nucleic acid region (A), the G-quartet is formed in the G-quartet-forming nucleic acid region (D) due to the contact, the G-quartet-forming region (D) and porphyrin forms a complex, and the complex generates fluorescence.

Fluorescence sensor for target analysis, kit for target analysis, and target analysis method using same

The present invention provides a novel sensor for detecting streptavidin (SA). The nucleic acid sensor for analyzing SA of the present invention includes the following nucleic acid element that includes a catalyst nucleic acid molecule (D) that exerts a catalytic function and a binding nucleic acid molecule (A) that binds to SA. The nucleic acid element is a double-stranded nucleic acid element including a first strand and a second strand. The first strand (ss1) includes the binding nucleic acid molecule (A), a loop-forming sequence (L1), and the catalyst nucleic acid molecule (D) linked in this order. The second strand (ss2) includes a stem-forming sequence (S), a loop-forming sequence (L2), and a stem-forming sequence (S) linked in this order. In this nucleic acid element, in the absence of SA, the catalytic function of the catalyst nucleic acid molecule (D) is inhibited by stem formation in each of the stem-forming sequences (S) and (S), and in the presence of SA, the stem formation is released by a binding of the binding nucleic acid molecule (A) with the SA, and the catalytic function of the catalyst nucleic acid molecule (D) is exerted.

Device and method for analyzing streptavidin

The present invention provides a novel sensor for detecting a target. The nucleic acid sensor of the present invention includes a nucleic acid element that includes a catalyst nucleic acid molecule (D) that exerts a catalytic function and a binding nucleic acid molecule (A) that binds to a target. The nucleic acid element is a double-stranded nucleic acid element including a first strand and a second strand. The first strand (ss1) includes the binding nucleic acid molecule (A), a loop-forming sequence (L1), and the catalyst nucleic acid molecule (D) linked in this order. The second strand (ss2) includes a stem-forming sequence (S), a loop-forming sequence (L2), and a stem-forming sequence (S) linked in this order. In this nucleic acid element, in the absence of a target, the catalytic function of the catalyst nucleic acid molecule (D) is inhibited by stem formation in each of the stem-forming sequences (S) and (S), and in the presence of ATP/target, the stem formation is released by a binding of the binding nucleic acid molecule (A) with the target, and the catalytic function is exerted.

Device and method for analyzing target

The present invention provides a novel nucleic acid element candidate molecule for use in screening for a nucleic acid element for target analysis and a novel screening method for screening for a nucleic acid element for target analysis using the same. The candidate molecule according to the present invention is a molecule for screening for a nucleic acid element for target analysis, being the following single-stranded nucleic acid (I): (I) a single-stranded nucleic acid comprising a catalyst sequence (D), a blocking sequence (B), and a binding sequence (A) that binds to a target, linked in this order. The blocking sequence (B) is complementary to a partial region (Dp) in the catalyst sequence (D). A terminal region (Ab) on the blocking sequence (B) side in the binding sequence (A) is complementary to a flanking region (Df) of the partial region (Dp) in the catalyst sequence (D) and is complementary to a terminal region (Af) on the side opposite to the blocking sequence (B) side in the binding sequence (A).

Nucleic acid element candidate molecule and screening method for screening for nucleic acid element for target analysis using the same

The present invention provides a novel nucleic acid molecule that can be used for detection of peanuts. The peanut-binding nucleic acid molecule of the present invention is characterized in that it binds to a peanut allergen with a dissociation constant of 10 nM or less, and preferably contains a polynucleotide consisting of any of base sequences of SEQ ID NOs: 1 to 15, for example. It is also preferable that, for example, the peanut-binding nucleic acid molecule of the present invention binds with significant specificity to the peanut allergen rather than to a soybean protein.

Growing community of inventors

Tokyo, Japan

Katsunori Horii