Nitto-denko Corporation

Katsuji Tani

Shuji Senda

Akio Iwama

Yoshitaka Kazuse

Takeshi Saika

Tetsuji Sugii

Masao Nasu

Nobuyasu Yamaguchi

A pressure sensitive adhesive sheet for the detection of microorganisms, which comprises a laminate of an adhesive layer mainly composed of a water-soluble polymer and a water-permeable membrane which does not allow passage of the microorganisms; a pressure sensitive adhesive sheet for the detection of microorganisms, wherein the surface of the adhesive layer has a contact angle with water of not more than 90&deg;; and a method for detecting a microorganism, which comprises bringing the surface of an adhesive layer of a pressure sensitive adhesive sheet into contact with the surface of a test object, and then bringing the surface of the adhesive layer into contact with water, said adhesive layer or water containing a chromogenic reagent. The use of the laminate makes it possible to leave only stained microorganisms on the water-permeable membrane and to precisely observe the color developed by the microorganisms. The method of the present invention enables real-time monitoring without culture of microorganisms, and is not limited to the detection of viable cells alone. Inasmuch as a contact of the pressure sensitive adhesive sheet with a test face results in accumulation of microorganisms, the manipulation is simple. In addition, by properly determining a chromogenic reagent, a specific microorganism alone can be detected and counted, so that it can be applied to environmental investigation in the medical field, food industry field and the like.

Pressure sensitive adhesive sheet for detection of microorganism and method for detection of microorganism

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Takatsuki, Japan

Katsuji Tani