Tokyo Women's Medical University

Asahi Kasei Kabushiki Kaisha

Able Corporation

Metcela, Inc.

Katsuhisa Matsuura

Tatsuya Shimizu

Teruo Okano

Takahiro Iwamiya

Masanori Wada

Hiroyuki Tsurii

Kanako Konishi

Michi Sato

Ushio Iwamoto

Hiroyoshi Seta

The purpose of the present invention is to provide a cardiac cell culture material which specifically acts on cardiac cells. In addition, another purpose of the present invention is to provide artificial organ material obtained by culturing by using said cardiac cell culture material, and a method for producing the same. Thus, provided is a cardiac cell culture, wherein functional cardiac tissue is favorably built by using a cardiac cell culture material containing VCAM-1.

Cardiac cell culture material

A cell culture system having a cell culture vessel, a composition controlling fluid storage vessel, a culture fluid composition controlling means having an inlet and an outlet for a cell culture fluid, an inlet-connected fluid feeding circuit from the cell culture vessel to the inlet of the culture fluid composition controlling means, an outlet-connected fluid feeding circuit from the cell culture vessel to the outlet of the culture fluid composition controlling means, a means which perfuses the cell culture fluid from the inlet-connected fluid feeding circuit to the outlet-connected fluid feeding circuit through the culture fluid composition controlling means, and a means which controls the amount of fluid in the cell culture vessel, in which compositions of the cell culture fluid in the cell culture vessel and compositions of the composition controlling fluid in the composition controlling fluid storage vessel can be controlled in a continuous manner.

Cell culture system and cell culture method

The present invention pertains to a method for culturing a cell population including pluripotent stem cells and differentiated cells derived from pluripotent stem cells at a temperature of 40.5&#xb0; C. or higher and reducing the pluripotent stem cells included in the cell population. The present invention also pertains to a method for reducing pluripotent stem cells from a cell population including pluripotent stem cells and differentiated cells derived from pluripotent stem cells, wherein the method includes a step for activating the TRPV-1 expressed in the pluripotent stem cells included in the cell population. The present invention makes it possible to reduce the pluripotent stem cells remaining in an undifferentiated state when inducing the differentiation of a pluripotent stem cell population.

Method for reducing pluripotent stem cells, method for producing cell population having reduced pluripotent stem cells

Disclosed herein is a cell culture apparatus that can achieve appropriate culture conditions. The cell culture apparatus () includes: a cylindrical culture vessel () that holds a culture liquid containing cells; a supporting column () that stands upright in a center of an inner surface of a bottom () in the culture vessel; and a stirring device () that includes an attaching portion () that is attached to an upper portion of the supporting column so as to be rotatable relative to the supporting column and a stirring blade () whose upper portion is fixed to the attaching portion so as to rotate around the supporting column.

Cell culture apparatus and cell culture method using the same

Growing community of inventors

Tokyo, Japan

Katsuhisa Matsuura