National Institute of Advanced Industrial Science and Technology

Chisso Corporation

Agency of Industrial Science and Technology

Japan Chemical Innovation Institute

Jnc Corporation

Katsuhiko Ueno

Kazunori Kataoka

Noriyuki Ohnishi

Hirotaka Furukawa

Kazumi Aoshima

Mikiko Yoshida

The present invention relates to magnetic fine particles having a lower critical solution temperature to which at least one substance selected from biotin and avidin is immobilized, and a method of converting a substance, a method of separating or concentrating a microorganism, a method of modifying a denatured protein, a method of detecting a nucleic acid, a separating agent, and a method of separating a biological substance using the same.

Magnetic fine particles having lower critical solution temperature

A stimuli-responsive polymer derivative utilizing keto-enol tautomerization. Also disclosed are a simple process for producing an N-acyl(meth)acrylamide derivative which can be used as a monomer for the stimuli-responsive polymer, a process for the production of an intermediate thereof, and an intermediate thus produced.

Stimuli-responsive polymer utilizing keto-enol tautomerization and stimuli-responsive separating material and chemical-releasing capsule comprising the same

The present invention relates to magnetic fine particles having immobilized thereto a polymer having an upper critical solution temperature, and a process for producing the same, a method of separating or concentrating a microorganism, a method of purifying, detecting, or concentrating a nucleic acid, a separating agent, a method of separating a biological substance, and a method of converting a substance.

Magnetic fine particles and process for producing the same

The invention relates to a stimulus-responding polymer obtained by polymerizing at least a monomer represented by a general formula (1) and a monomer represented by a general formula (2), and a separation method or concentration method of microorganisms, a purification method, detection method or concentration method of nucleic acids, a separating agent, a separation method of biomaterials and a conversion method of materials, which use this polymer, (in the formula, Rrepresents hydrogen atom or methyl group, and Rrepresents single bond or a straight or branched alkylene group having from 1 to 5 carbon atoms) (in the formula, Rrepresents hydrogen atom or methyl group, and Rrepresents hydrogen atom, a straight, branched or cyclic alkyl group, alkoxyl group or alkylamino group having from 1 to 10 carbon atoms, an aryl group or a heterocyclic group).

Polymer having an upper critical solution temperature

The present invention is to prepare a novel polymerizable biotin derivative represented by formula (I) shown below and make it possible to synthesize or design a polyfunctional or multifunctional polymer using the derivative. In formula (I), Rrepresents a hydrogen atom or an alkyl group, Rand Reach independently represents a hydrogen atom, an alkyl group or an aryl group, T represents an oxygen atom or &#x2550;NH, W represents a single bond, a carbonyl group, a thiocarbonyl group or a Calkylene group, U represents a single bond or &#x2014;NH&#x2014;, X represents a single bond, a Chydrocarbon bond, an oxygen atom or &#x2014;NH&#x2014;, Y represents a single bond, a carbonyl group, a thiocarbonyl group, &#x2014;NH&#x2014;, a 1,2-dioxyethylene group or a 1,2-diaminoethylene group, Z represents a single bond, a carbonyl group, a thiocarbonyl group, a Calkylene group, an oxygen atom or &#x2014;NH&#x2014;, and V represents a single bond or a Calkylene group.

Polymerizable biotin derivatives, biotin polymer, and polymer responsive to avidin stimulation

The problem of the invention is to find a polymer mixture which forms an inter-polymer complex by being responsive to temperature even under neutral to alkaline conditions. According to the invention, a temperature responsive inter-polymer complex capable of showing thermal responsiveness in an aqueous solution, which contains a poly-N-acetylacrylamide or polyvinyl alcohol and polyethylene glycol, polyacrylamide or polymethacylamide, is applied to separating agents, immobilized enzymes, denatured protein modifiers, separation method or concentration of microorganisms, purification or concentration of nucleic acids, drug-releasing microcapsules and the like.

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Tsukuba, Japan

Katsuhiko Ueno