Ajinomoto Co., Ltd.

National Institute of Advanced Industrial Science and Technology

The University of Tokyo

Riken Corporation

National Institute of Technology and Evaluation

National Research Institute of Brewing

Mitsukan Group Corporation

Katsuhiko Kitamoto

Kyoko Koibuchi

Junichi Maruyama

Hiroaki Nagasaki

Jiro Kataoka

Ari Yuasa

Manabu Arioka

Kiyoshi Asai

Shotaro Yamaguchi

Yoichi Ueda

Masayuki Machida

Keiko Abe

Keietsu Abe

Katsuya Gomi

Motoaki Sano

Satoru Kuhara

Naotake Ogasawara

Hideki Nagasaki

Akira Hosoyama

Taishin Kin

Osamu Akita

Tomiko Asakura

Shigeharu Moriya

Daiki Ninomiya

Jun-ichi Maruyama

Toshiaki Kudo

Mari Kojima

Mikiya Kishi

Kenichiro Nakajima

Tazuko Uenoyama

Hiroyuki Sorimachi

Nemuri Todaka

Tetsushi Inoue

Hideki c/o Aist Tokyo Waterfront Nagasaki

Shotaro c/o Gifu R & D Center Yamaguchi

Motoaki c/o Tsukuba Central 6 Sano

Masayuki c/o Tsukuba Central 6 Machida

Kiyoshi c/o Aist Tokyo Waterfront Asai

Taishin c/o Aist Tokyo Waterfront Kin

This invention provides: DNA encoding cellulase enzymes from intestinal symbiotic protists of the insects of, and Cryptocercidae, comprising the nucleotide sequences as shown in SEQ ID NOS:1 to 140; an expression system for the DNA; and a method for producing the cellulase enzymes using the expression system.

Polynucleotide encoding a cellulase enzyme and method for producing the enzyme

Disclosed is a polypeptide shown in the following in (A), and a protein dimer neoculin comprising the polypeptide Neoculin Acidic Subunit (NAS) and the polypeptide Neoculin Basic Subunit (NBS) and having a taste-modifying activity:

DNA encoding a neoculin acidic subunit of curculin

It is intended to provide koji mold-origin phospholipase Aand a DNA encoding it. Namely, phospholipase Acomprising the following protein (a) or (b): (a) a protein having an amino acid sequence represented by SEQ ID NO: 1 or 2; and (b) a protein having an amino acid sequence derived from an amino acid sequence represented by SEQ ID NO: 1 or 2 by a partial modification and serving as phospholipase A.

[object Object]

Glutaminase is purified from an, its partial amino acid sequence is determined, a partial sequence of glutaminase gene is obtained by PCR based on the obtained information, and DNA fragments containing glutaminase gene fromgenomic library and cDNA library, andgenomic library by hybridization using the partial sequence as a probe.

Glutaminase, its gene and a method of producing it

The object of the present invention is to provide Koji mold aminopeptidases capable of efficiently hydrolyzing persistent peptides and also genes encoding the aminopeptidases. The present invention providesaminopeptidase and nucleic acid molecules encoding it. In particular, the present invention provides a protein having an amino acid sequence represented by amino acid Nos. 1 to 519 in SEQ ID NO: 2, or a protein containing the substitution, deletion, insertion, addition or inversion of one or more amino acids in said sequence, and which protein has an activity of catalyzing the reaction for releasing an amino acid at an N-terminal of a peptide, and nucleic acid molecules encoding them.

Aminopeptidase and the genes thereof

Glutaminase is purified from an , its partial amino acid sequence is determined, a partial sequence of glutaminase gene is obtained by PCR based on the obtained information, and DNA fragments containing glutaminase gene from genomic library and cDNA library, and genomic library hybridization using the partial sequence as a probe.

Growing community of inventors

Tokyo, Japan

Katsuhiko Kitamoto