Kyowa Hakko Kogyo Co., Ltd.

Kyowa Hakko Kogyo Kabushiki Kaisha

Kyowa Hakko Kogyo

Kyowa Hakko Kabushiki Kaisha

Katsuhiko Ando

Keiko Ochiai

Akio Ozaki

Hideo Mori

Hiroshi Sano

Isao Kawamoto

Shigeru Chiba

Takeshi Shibasaki

Hiroshi Kase

Toru Yasuzawa

Koji Yamada

Yuzuru Matsuda

Yoichi Uosaki

Mayumi Yoshida

Joji Goto

Yoshinori Yamashita

Hirofumi Nakano

Satoshi Nakanishi

Tsutomu Agatsuma

Chiaki Saitoh

Yutaka Saitoh

Kazuo Aisaka

Isami Takahashi

Takeo Tanaka

Hidemasa Kondo

Noriaki Hirayama

Fumito Koizumi

Etsuyo Shimizu

Yutaka Saito

Shiro Akinaga

Akira Asai

Ryoichi Katsumata

Tamio Mizukami

Tatsuya Tamaoki

Toru Mizukami

Hiroaki Motoyama

Kozo Ouchi

Masaru Hasegawa

Nobuo Ogata

Yutaka Takano

Katsunori Kita

Atsushi Yashiro

Hideyo Kumazawa

Chikara Tokunaga

Kyoko Ozawa

Atsuko Yokoi

Hiroshi Katahira

Kazutoshi Kuroda

Mitsuru Takahashi

Yasuhiro Suzuki

Kazuhiro Kubo

Tadashi Ashizawa

Eiji Tsukuda

Eiji Kobayashi

Katsushige Gomi

Katsuichi Shuto

Takao Iida

Keiichi Takahashi

Mitsunobu Hara

Youichi Uosaki

Ryuichiro Nakai

Hideki Kawasaki

Akira Mihara

Hiroki Ishiguro

Harumi Ogawa

Hiroyuki Nagata

Shingo Kakita

Yoshikazu Morishita

Junichi Ikeda

Noboru Fujii

Sakae Tazoe

Toshiaki Iwazaki

Yumiko Aotani

Yasushi Nishiie

Naoki Morishima

Tomoyuki Sano

Mika Nozawa

Shinichiro Toki

Hiroe Ohno

The present invention is directed to a process for producing trans-4-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into trans-4-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genusorand which catalyzes the hydroxylation of L-proline into trans-4-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced trans-4-hydroxy-L-proline is collected from the aqueous medium. In addition, the present invention is directed to a process for producing trans-4-hydroxy-L-proline, wherein the L-proline biosynthesis activity of the host cell of the transformant is reinforced.

Process for producing trans-4-hydroxy-L-proline

An isolated gene encodes for a L-proline-3-hydroxylase having the amino acid sequence of SEQ ID NOS: 1, 2, 15, 16 and 17, or for a protein having enzymatic activity to hydroxylate the 3-position of L-proline and to act on free L-proline in the presence of 2-ketoglutaric acid and divalent iron ions to produce cis-3-hydroxy-L-proline. The gene is inserted into a vector and the resulting recombinant DNA is used to create a transformant. L-proline-3-hydroxylase is produced by culturing the transformant in a medium.

Process for producing cis-3-hydroxy-L-proline

The present invention relates to a novel flavoring solution for making pickles having a flavor of foods pickled in rice bran paste (hereinafter referred to as a bran pickles flavoring solution), a clear bran pickles flavoring solution, processes for producing said solutions, a novel microorganism belonging to the genusused for the production of said flavoring solutions, and a process for producing a solution containing a lactone wherein the lactone is selectively extracted from a culture of a lactone-producing microorganism. The present invention also relates to a novel bacterium of the genuswhich has the ability to produce &#x3b3;-dodecalactone and/or &#x3b3;-dodecelactone and is useful for the production of said bran pickles flavoring solutions.

Process for producing a bran pickles flavoring solution

Provided is an industrially applicable process for producing trans-4-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into trans-4-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Dactylosporangium, Amycolatopsis or Streptomyces and which catalyzes the hydroxylation of L-proline into trans-4-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced trans-4-hydroxy-L-proline is collected from the aqueous medium. Also provided is a novel enzyme L-proline-4-hydroxylase which is useful for the process, a gene of L-proline-4-hydroxylase which is useful for the process, a transformant containing the gene, and a process for producing L-proline-4-hydroxylase using the transformant. In addition, provided is a process for producing L-proline-4-hydroxylase using the transformant which contains the gene and has a reinforced proline biosynthesis activity.

CHOLESTEROL DEHYDROGENASE, COPROSTAN-3-ONE DEHYDROGENASE AND 4-CHOLESTEN-3-ONE DEHYDROGENASE, COMPOSITIONS CONTAINING THE DEHYDROGENASES, AND METHOD FOR REDUCING AMOUNT OF CHOLESTEROL USING THE COMPOSITIONS

Bacillus strains which produce HMG-CoA reductase inhibitors

An industrially applicable process for producing cis-3-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into cis-3-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Streptomyces or Bacillus and which catalyzes hydroxylation of L-proline into cis-3-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced cis-3-hydroxy-L-proline is collected from the aqueous medium. A novel enzyme L-proline-3-hydroxylase, a gene of L-proline-3-hydroxylase which is useful for the process, a transformant containing the gene, and a process for producing L-proline-3-hydroxylase using the transformant.

Process for producing CIS-3-hydroxy-L-proline

A flavoring solution for making pickles having a flavor of foods pickled in rice bran paste, a clear bran pickles flavoring solution, processes for producing the flavoring solutions, a process for producing lactones which are essential components of the flavoring solutions, and a bacterium belonging to the genus Corynebacterium which is useful for producing a lactone are disclosed. The bacterium is preferably Corynebacterium sp. NK-1 (FERM BP-6329). The lactone is preferably a &ggr; lactone.

Process for producing a lactone using corynebacterium sp. NK-1 (FERM BP-6329)

An enzymatic process is presented for reducing the cholesterol level of foods and feeds by converting the cholesterol therein to coprostanol which has a very low intestinal tract absorbability. Cholesterol in meat, egg, milk, seafood and cooked processed foods containing the same, or feeds for animals, poultry, and pisiculture can be reduced by the sequential action of three enzymes isolated from a eubacterium: cholesterol dehydrogenase, 4-cholesten-3-one dehydrogenase and coprostan-3-one dehydrogenase.

Process for producing a cholesterol-reduced substance

Disclosed is a process for producing a compound represented by general formula (II-a):

Process for the preparation of HMG-COA reductase inhibitors

An industrially applicable process for producing trans-4-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into trans-4-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Dactylosporangium, Amycolatopsis or Streptomyces and which catalyzes the hydroxylation of L-proline into trans-4-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced trans-4-hydroxy-L-proline is collected from the aqueous medium. Also provided is a novel enzyme L-proline-4-hydroxylase which is useful for the process, a gene of L-proline-4-hydroxylase which is useful for the process, a transformant containing the gene, and a process for producing L-proline-4-hydroxylase using the transformant. In addition, provided is a process for producing L-proline-4-hydroxylase using the transformant which contains the gene and has a reinforced proline biosynthesis activity.

Method for quantitative determination of 1,5-anhydroglucitol

An industrially applicable process for producing cis-3-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into cis-3-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Streptomyces or Bacillus and which catalyzes hydroxylation of L-proline into cis-3-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced cis-3-hydroxy-L-proline is collected from the aqueous medium. A novel enzyme L-proline-3-hydroxylase, a gene of L-proline-3-hydroxylase which is useful for the process, a transformant containing the gene, and a process for producing L-proline-3-hydroxylase using the transformant, is also provided.

Process for producing Cis-3-hydroxy-L-proline

UCT1072 compounds

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Machida, Japan

Katsuhiko Ando