Symrise Ag

Leibniz-institut Fur Pflanzenbiochemie

Katrin Geissler

Jakob Peter Ley

Torsten Geissler

Ludger A Wessjohann

Michael Backes

Holger Zorn

Jens-Michael Hilmer

Egon Gross

Fabia Hentschel

Martin Dippe

Susann Herrmann

Marco A Fraatz

Johanna Rost

Michael Goldmann

Anne-Kathrin Bauer

Marco Fraatz

The present invention relates to genetically modified enzymes obtained by rational design of the active site binding pocket of the prototypic enzyme 4-hydroxyphenylacetate 3-hydroxylase (4HPA3H) for hydroxylating a 4-hydroxyphenyl compound to yield a 3,4-dihydroxyphenyl compound and to biotechnological methods including in vivo and in vitro methods using said enzymes or catalytically active fragments thereof. Further provided is a method either using a suitable oxidase or hydroxylase further enabling the subsequent site specific methylation of the 3,4-dihydroxyphenyl compound in a coupled enzymatic reaction by providing a suitable O-methyltransferase. Finally, compositions obtainable by the aforementioned methods are disclosed.

Mutant 4-hydroxyphenylacetate 3-hydroxylases and uses thereof

A method for producing branched aldehydes is proposed, the method comprising the following steps:

Method for producing branched aldehydes

A method is proposed for producing flavonoids, comprising the steps: (a) providing of a transgenic microorganism, containing (i) a first nucleic acid section (A), comprising or consisting of a gene coding for a CYP450 oxidase, (ii) a second nucleic acid section (B), comprising or consisting of a gene coding for a plant O-methyltransferase, and (b) adding of one or more flavanones to the transgenic microorganism, (c) the conversion of the substrate flavanones by the transgenic microorganism to the corresponding flavonoids, and optionally (d) isolating and purifying of the final products.

Method for the biotechnological production of flavonoids

Growing community of inventors

Einbeck, Germany

Katrin Geissler