Boehringer Ingelheim Vetmedica Gmbh

Siemens Healthcare Diagnostics Gmbh

Siemens Aktiengesellschaft

Siemens Healthcare Gmbh

Katja Friedrich

Walter Gumbrecht

Peter Paulicka

Manfred Stanzel

Joachim Bangert

Karsten Hiltawsky

Renee Weber

A method is disclosed for calibrating a sensor element, which has an immobilized probe oligonucleotide, via which the bonding of a target nucleic acid (Z) can be detected by the sensor. In at least one embodiment, the method includes: a) bringing the sensor element into contact with a control nucleic acid (K), the melting temperature Tm(K) of which is less than the melting temperature Tm(Z) of the target nucleic acid (Z); b) hybridizing the control nucleic acid (K) to the probe oligonucleotide at a temperature T[p]&#x3c;Tm(K), and detecting a positive control signal; and optionally c) modifying the stringent conditions such that T[n]&#x3e;Tm(K) and detecting a negative control signal at a temperature T[n]. According to a refinement of at least one embodiment of the invention, a measuring signal of the target nucleic acid (Z) is measured at a measuring temperature T [mess], where Tm(K)&#x3c;T[mess]&#x3c;Tm(Z). The method is suited in particular for the calibration and quality control of microarrays.

Method and arrangement for calibrating a sensor element

A process is disclosed for detecting cells in a liquid sample, which includes: i) filtration of the liquid sample through a porous membrane which is suitable for retaining detectable cells, where at least one subregion of a support is configured as transparent supporting body and the membrane is arranged over its area on the transparent supporting body in such a way that detectable cells are retained on at least part of the surface of the membrane and that at least part of the sample liquid passes through the membrane, ii) application of a liquid optical medium which has essentially the same refractive index as the supporting body, and iii) optical measurement of at least a subarea of the membrane in order to detect detectable cells.

Arrangement and process for optical analysis and specific isolation of biological samples

An array of valves are arranged in n columns and m lines and which are each designed to control a fluid flow in an associated flow channel in a lab-on-a-chip system. The array includes at least two valves, every column having not more than one valve and every line having from zero to n valves. A device is used for actuating the valves. The valves are pressure-actuated. To produce the device, the flow channels are arranged in accordance with the arrangement of the valves. Which valves are actuated being changeable by movement of projections relative to array of valves so as to change which valves the projections are pressed against.

Device for controlling fluid flows in lab-on-a-chip systems

An array of valves are arranged in n columns and m lines and which are each designed to control a fluid flow in an associated flow channel in a lab-on-a-chip system. The array includes at least two valves, every column having not more than one valve and every line having from zero to n valves. A device is used for actuating the valves. The valves are pressure-actuated. To produce the device, the flow channels are arranged in accordance with the arrangement of the valves.

Device and method for controlling fluid flows in lab-on-a-chip systems and method for producing device

A substrate of a lab-on-a-chip system has two adjacent recesses, one serving as a flow channel and the other one being filled with an elastomer compound. In a first state, the elastomer compound and the substrate delimit the flow channel in a section. In a second state, the elastomer compound takes up the space in the recess in the substrate along a cross-section of the flow channel, thereby completely closing the flow channel. The substrate and the elastomer compound may be produced by injection molding techniques.

Growing community of inventors

Nürnberg, Germany

Katja Friedrich