Dako Denmark A/s

Katja Brunstedt

Henrik Pedersen

Jesper Lohse

Liselotte Brix

Jørgen Schøller

Tina Brink Jakobsen

Kivin Jacobsen

Tina Jakobsen

The present invention relates to a soluble negative control MHC multimer comprising a nonsense peptide that binds the MHC protein efficiently, but that does not support binding of the resultant MHC-peptide complex to the desired T Cell Receptor. The nonsense peptide is designed to i) have a length enabling binding to the MHC allele in question, ii) have appropriate amino acids at relevant anchor positions which anchor the nonsense peptide to the peptide-binding groove of the MHC, iii) have amino acids outside the anchor positions that do not support binding to a T Cell Receptor, and iv) have an amino acid sequence that is different from the linear sequence of any naturally occurring peptide.

MHC multimers, methods for their generation, labeling and use

Growing community of inventors

Lyngby, Denmark

Katja Brunstedt