Fluidigm Corporation

Syngen, Inc.

Kathy Yokobata

Andrew Paul May

Hou-Pu Chou

Michael Ybarra Lucero

Hany Ramez Nassef

Ronald H Chiarello

Wing Liu

A microfluidic device adapted to perform many simultaneous binding assays including but not limited to immunological experiments, such as ELISA assays, with minimal cross-talk between primary and secondary antibodies.

Microfluidic assay devices and methods

Fluorescent dyes based on rhodamine are derivatized to form labeled conjugates that fluoresce upon excitation with light of an appropriate wavelength. Particularly preferred embodiments are certain single isomer form rhodamine phosphoramidites. These rhodamine phosphoramidites enhance the efficiency of synthesizing rhodamine-labeled oligonucleotides by solid phase methods. Conjugate embodiments of the invention are prevented from being converted to a non-fluorescent lactam form due to having a fully substituted amide linkage derived from the 3-position carboxylate.

Rhodamine-based fluorophores useful as labeling reagents

Growing community of inventors

Sunnyvale, CA, United States of America

Kathy Yokobata