Corning Incorporated

Kathy Marie Youngbear

Joseph Christopher Wall

John A Ryan

Nikolaos Pantelis Kladias

William Joseph Lacey

David A Kenney

Todd M Upton

Aravind Raghavan Rammohan

Ma Sha

Shang-Pin Kwei

Stephen R Bossi

Laurence M Vaughan

David Alan Kenney

A 5-liter bioreactor vessel enables a substantial increase in the volume of biological media that can be cultivated in the vessel compared to conventional designs. Moreover, when agitated at 1.5&#xd7; the shaking frequency of a conventional 3-liter bioreactor (i.e., 90 rpm versus 60 rpm), the 5-liter vessel achieves a 19% increase in cell aeration without exceeding the maximum shear stress limit for cell viability. The 5-liter vessel optionally includes a plurality of internal baffles configured to disrupt the liquid vortex and reduce the maximum shear stress transferred to biomedia contained within the vessel.

Vessel for growth of biological entities

The present invention relates generally to a cell culture stirring vessel and associated impeller. More particularly, it relates to a fully integrated, disposable spinner flask vessel having a suspended impeller assembly permanently integrated therein. The invention is particularly suitable for use in applications where cells are suspended within a liquid medium with minimal shear forces.

Disposable spinner flask

A stackable flask for the culturing of cells is disclosed. The cell culture chamber is defined by a top plate and a rigid bottom tray of substantially rectangular shape connected by side and end walls, the body of the flask has imparted therein an opening connected to an angled neck, the neck being hydrophobic to keep fluid out. The neck is also modified to accept a snap-fit cap with a modified stepped skirt. The stepped skirt further prevents contamination by protecting the inner mating surface from contacting a surface. The size of the flask and location of an optional separate neck and cap section allows for flask manipulation by standard automated assay equipment, making the flask ideal for high throughput applications.

Cell cultivating flask

A stackable flask for the culturing of cells is disclosed. The cell culture chamber is defined by a top plate and a rigid bottom tray of substantially rectangular shape connected by side and end walls, the body of the flask has imparted therein a gas permeable membrane that will allow the free flow of gases between the cell culture chamber and the external environment. The flask body also includes a sealed septum that will allow access to the cell growth chamber by means of a needle or cannula. The size of the flask and location of an optional neck and cap section allows for flask manipulation by standard automated assay equipment, making the flask ideal for high throughput applications.

The present invention includes a microplate for performing crystallography studies and methods for making and using such microplates. In particular, the microplate has a frame which includes a plurality of wells formed therein. Each well includes a first well and a second well. The first well includes a relatively small reservoir having a substantially concaved form capable of receiving a protein solution and a reagent solution. The second well includes a relatively large reservoir capable of receiving a reagent solution that has a higher concentration than the reagent solution within the first well, wherein the protein solution and the reagent solution within the first well interact with the reagent solution within the second well via a vapor diffusion process which enables the formation of protein crystals within the first well. The microplate may be sized so that it can be handled by a robotic handling system and/or a liquid handling system.

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Cambridge, MA, United States of America

Kathy Marie Youngbear