Illumina Corporation

Kathryn M Stephens

Min-Jui Richard Shen

Bala Murali Venkatesan

Kevin L Gunderson

Jonathan Mark Boutell

M Shane Bowen

Kandaswamy Vijayan

Mostafa Ronaghi

Anne Jager

Frank J Steemers

Sasan Amini

Cydne Holt

Paulina Walichiewicz

Yonmee Han

Marcus Burch

Carey Davis

David Silva

Li Liu

Shannon Kaplan

Sean M Ramirez

Rigo Pantoja

Justin Fullerton

Alex So

Steven Modiano

Timothy J Wilson

Neeraj Salathia

Timothy Wilson

Frank Steemers

Embodiments disclosed herein provide methods for constructing a DNA profile comprising: providing a nucleic acid sample, amplifying the nucleic acid sample with a plurality of primers that specifically hybridize to at least one target sequence comprising a SNP and at least one target sequence comprising a tandem repeat, and determining the genotypes of the at least one SNP and at least one tandem repeat in the amplification products, thereby constructing the DNA profile of the nucleic acid sample. Embodiments disclosed herein further provide a plurality of primers that specifically hybridize to at least one short target sequence and at least one long target sequence in a nucleic acid sample, wherein amplifying the nucleic acid sample using the plurality of primers in a single reaction results in a short amplification product and a long amplification product, wherein each of the plurality of primers comprises one or more tag sequences.

Methods and compositions for DNA profiling

A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.

Kinetic exclusion amplification of nucleic acid libraries

Presented are methods and compositions for spatial detection and analysis of nucleic acids in a tissue sample. The methods can enable the characterization of transcriptomes and/or genomic variations in tissues while preserving spatial information about the tissue.

Spatial mapping of nucleic acid sequence information

The present system provides novel methods and compositions for selecting a particular strand of RNA and/or producing a cDNA library that results in an unbiased representation of RNA in a sample.

Methods for producing stranded cDNA libraries

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Poway, CA, United States of America

Kathryn M Stephens