Darwin Discovery Limited

Ucb S.a.

Decode Biostructures, Inc.

Kathryn Hjerrild

Mary E Brunkow

Fred Ramsdell

Eric W Jeffery

Lansing J Stewart

Alex Burgin

John W Walchli

Pieces of software and databases are used to facilitate the design and synthesis of genes. The synthesis techniques allow identification, quantification, transcription, translation, and manipulation of portions of the gene sequence represented as 0's and 1's in a computing system. These pieces of software and databases compile constraint information provided by a user to create synthetic genes to express proteins lacking disordered or variable regions and containing surface mutations that promote solubility and/or crystallization. Regions of the gene sequence that do not form stable structures can be removed and replaced by a short biological linker to improve crystallization of proteins.

Gene synthesis software

Isolated nucleic acid molecules are provided which encode Fkh, as well as mutant forms thereof. Also provided are expression vectors suitable for expressing such nucleic acid molecules, and host cells containing such expression vectors. Utilizing assays based upon the nucleic acid sequences disclosed herein (as well as mutant forms thereof), numerous molecules may be identified which modulate the immune system.

Identification of the gene causing the mouse scurfy phenotype and its human ortholog

Isolated nucleic acid molecules are provided which encode Fkh , as well as mutant forms thereof. Also provided are expression vectors suitable for expressing such nucleic acid molecules, and host cells containing such expression vectors. Utilizing assays based upon the nucleic acid sequences disclosed herein (as well as mutant forms thereof), numerous molecules may be identified which modulate the immune system.

Isolated nucleic acid molecules are provided which encode Fkh , as well as mutant forms thereof. Also provided are expression vectors suitable for expressing such nucleic acid molecules, and host cells containing such expression vectors. Assays that are based upon the nucleic acid sequences disclosed herein (as well as mutant forms thereof), may be used for identifying numerous molecules that modulate the immune system. Molecules that specifically bind to an Fkh polypeptide are also provided as useful, such as, for modulating the biological activities of Fkh . In particular, antibodies that are specific for Fkh polypeptide are provided for uses such as for detecting the expression of an Fkh polypeptide in cells, isolating an Fkh polypeptide, and modulating the activity of an Fkh polypeptide.

Antibodies specific for Fkhsf gene products

Isolated nucleic acid molecules are provided which encode Fkh , as well as mutant forms thereof. Also provided are expression vectors suitable for expressing such nucleic acid molecules, and host cells containing such expression vectors. Utilizing assays based upon the nucleic acid sequences disclosed herein (as well as mutant forms thereof), numerous molecules may be identified which modulate the immune system

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Bainbridge Island, WA, United States of America

Kathryn Hjerrild