Inguran, LLC

Kathleen S Crowley

Gary Durack

Jeremy T Hatcher

Cindy L Ludwig

Jeffrey D Wallace

Gary P Vandre

Lon A Westfall

Jeffrey Alan Graham

Muhammad Anzar

Bradley Didion

Charles N Graves

Jeffrey A Graham

David R Helbing

A method of cryopreserving sperm cells which may include cooling the quantity of sperm cells to a holding temperature, maintaining the sperm cells substantially at the holding temperature for a period, and supercooling the sperm cells by cooling the sperm cells at a first cooling rate to a temperature that approaches a critical temperature zone at which ice crystal formation and changes in osmotic pressure damage sperm cells and then cooling the sperm at a second cooling rate faster than said first cooling rate through said critical temperature zone.

Methods for processing sperm cells

A method of processing animal sperm cells that includes: collecting sperm cells from a male animal; sorting the sperm cells to obtain a quantity of sperm cells having a desired characteristic, the quantity of sorted sperm cells being contained in a first volume of fluid having a first concentration of sperm cells; and subjecting the sperm cells to concentration steps in which the concentration of the sperm cells is increased to a second concentration greater than the first concentration.

Sperm cell processing methods

Staining mixtures comprising viable spermatozoa, a composition which regulates oxidation/reduction reactions intracellularly or extracellularly, and a DNA selective dye are disclosed. The cells contained in such suspensions tend to have a greater capacity for enduring the various process steps typically associated with the sorting of sperm cells into gender enriched populations, thereby resulting in post-sort compositions with an increased number of viable or motile sperm. Processes for staining sperm cells comprising the formation of a staining mixture are also disclosed.

Use of a composition which regulates oxidation/reduction reactions intracellularly and/or extracellulary in a staining or sorting process

Processes of storing sorted and unsorted spermatozoa, in the form of a sperm dispersion, having reduced motility relative to endogenous ejaculated sperm are disclosed. The immotile spermatozoa of the dispersion tend to have a greater capacity for enduring the rigors associated with storage, transportation, and fertilization procedures. Processes for providing such sperm dispersions, for inseminating a female mammal with such sperm dispersions, as well as compositions and combinations comprising the sperm dispersions, are also disclosed.

Process for storing a sperm dispersion

Sperm cell suspensions comprising a motility inhibitor are disclosed. The cells contained in such suspensions tend to have a greater capacity for enduring the various process steps typically associated with the sorting of sperm cells into gender enriched populations, thereby resulting in post-sort compositions with an increased number of viable or motile sperm. Processes for forming such cell suspensions, as well as processes for staining sperm cells, are also disclosed.

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Webster Groves, MO, United States of America

Kathleen S Crowley