Plant Genetic Systems N.v.

Bayer Cropscience Nv

Bayer Cropscience Ag

Bayer Cropscience Gmbh

Basf Agricultural Solutions Seed US LLC

Bayer Bioscience N.v.

Aventis Cropscience, N.v.

Kathleen D'Halluin

Elke Gobel

Rene Ruiter

Jan Dittgen

Udo Matthias Bickers

Frank Meulewaeter

Stefan K Jansens

Ruediger Hain

Linda Trolinder

Thomas Wilde

Hal Moser

Chantal Vanderstraeten

Koen Weterings

Gary Henniger

Methods and means are provided to modify in a targeted manner the genome of a plant in close proximity to an existing elite event using a double stranded DNA break inducing enzyme. Also provided are plants, in particular cotton plants showing tolerance to a field dose of at least 1X of at least one HPPD inhibitor, and methods for making such plants.

Methods and means to modify a plant genome

Methods and means are provided to modify in a targeted manner the genome of a plant in close proximity to an existing elite event using a double stranded DNA break inducing enzyme. Also provided are plants, in particular cotton plants showing tolerance to a field dose of at least 1&#xd7; of at least one HPPD inhibitor, and methods for making such plants.

Methods and means are provided to modify in a targeted manner the plant genome of transgenic plants comprising chimeric genes wherein the chimeric genes have a DNA element commonly used in plant molecular biology. Re-designed meganucleases to cleave such an element commonly used in plant molecular biology are provided.

Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering

Alternative and/or improved methods are described for the exact removal of a selected subfragment from a DNA molecule by intrachromosomal recombination between two directly repeated DNA sequences using a rare-cleaving double stranded break inducing DNA endonuclease expressed under control of a micro-spore specific promoter. These methods can be applied for the exact exchange of a target DNA fragment for a DNA fragment of interest in plant cells and plants.

Methods and means for removal of a selected DNA sequence

A method is described for the exact removal of a selected subfragment from a DNA molecule by intrachromosomal recombination between two directly repeated DNA sequences using a rare-cleaving double stranded break inducing DNA endonuclease expressed under control of a micro-spore specific promoter. This method can be applied in a method for the exact exchange of a target DNA fragment for a DNA fragment of interest in plant cells and plants.

Methods and means are provided to improve targeted DNA insertion in plants using rare-cleaving 'double stranded break' inducing enzymes. Also provided are improved I-SceI encoding nucleotide sequences.

Targeted DNA insertion in plants

Transformation method for plants

A process for integrating a DNA fragment into the genome of a cell of a monocotyledonous plant, the process comprising the steps of:

A process for the stable integration of a DNA, comprising a gene that is function in a cell of a cereal plant, wherein said DNA is integrated into the nuclear genome of said cereal plant, said process comprising the steps of:

Process for transforming monocotyledonous plants

The present invention provides a rapid and efficient method for transforming monocotyledonous plants. The invention particularly relates to the use of either intact tissue capable of forming compact embryogenic callus or compact embryogenic callus obtained from such tissue to obtain transgenic monocotyledonous plants. The present invention also provides novel transgenic plants obtained by the transformation method of the present invention.

A process for the stable integration of a DNA comprising a gene that is functional in a cell of a cereal plant wherein the DNA is integrated into the nuclear genome of a cereal plant, the process comprising:

Growing community of inventors

Mariakerke, Belgium

Kathleen D'Halluin