Dow Agrosciences LLC

Katherine Armstrong

Otto Folkerts

Kelley Ann Smith

Dayakar Reddy Pareddy

Nicole Linda Hopkins

Aaron Todd Woosley

Joseph F Petolino

Timothy Denver Hey

Neil Madison Cowen

Beth C Rubin-Wilson

Michael A Menke

Scott Belmar

Michael Ainley

Lining Guo

Scott A Young

Tom Skokut

Kelley A Smith

The present invention provides DNA sequences that function as 3' untranslated regions (3&#x2032;UTR) in plants. The 3&#x2032;UTR's stabilize associated recombinant transcripts such that expression is improved. The invention further provides plant expression cassettes and recombinant plant that comprise a claimed 3&#x2032;UTR.

Use of regulatory sequences in transgenic plants

The maize myo-inositol-1-phosphate synthase (MIP synthase) promoter is a novel embryo specific regulatory sequence useful in expressing heterologous nucleic acids in plants.

Maize MIP synthase promoter

Cotton embryogenic callus tissues can be transformed by elongated, needle-like structures called &ldquo;whiskers&rdquo;. The process comprises the agitation of plant cell aggregates and plant tissues of the plant to be transformed in the presence of DNA and whiskers, whereby DNA uptake and integration thereof is facilitated.

Whisker-mediated transformation of cotton embryogenic callus tissues and regeneration of plants thereof

Regulatory sequences derived from the maize per5 gene have utility in plant biotechnology.

Regulatory sequences for transgenic plants

This invention relates to compositions and methods useful in the production of transgenic plants. In particular, the invention relates to maize metallothionein gene transcription regulatory sequences which are useful for directing expression of heterologous DNAs in plant embryo tissue. The invention also relates to expression cassettes containing the promoter sequences and to transgenic plants containing the expression cassettes.

Regulatory sequences useful for gene expression in plant embryo tissue

Regulatory sequences derived from a maize root preferential cationic peroxidase gene (Per5), including the promoter, introns, and the 3&prime; untranslated region, are useful to control expression of recombinant genes in plants.

Plant cell aggregates and plant tissues can be transformed by elongated, needle-like structures called &ldquo;whiskers&rdquo;. The process comprises the agitation of plant cell aggregates and plant tissues of the plant to be transformed in the presence of DNA and whiskers, whereby DNA uptake and integration thereof is facilitated. The process may be applicable to other plant cell aggregates and plant tissues which have not proven easily transformable by other techniques.

Transcriptional regulatory region

Genes encoding maize oleoyl-ACP and palmitoyl-ACP thioesterase enzymes have been isolated from maize. These genes, when expressed in a plant, can be used to create transgenic plants having altered palmitic acid oil profiles.

Growing community of inventors

Zionsville, IN, United States of America

Katherine Armstrong