Instytut Biochemii I Biofizyki Pan

Instytut Biotechnologii I Anty-biotykow

Siec Badawcza Lukasiewicz—instytut Biotechnologii I Antybiotykow

Uniwersytet Gdanski

Siec Badawcza Lukasiewicz-instytut Chemii Przemyslowej Imienia Profesora Ignacego Moscickiego

Katarzyna Florys

Violetta Sączyńska

Piotr Borowicz

Violetta Cecuda-Adamczewska

Boguslaw Szewczyk

Piotr Baran

Zenon Minta

Krzysztof Śmietanka

Andrzej Plucienniczak

Iwona Sokolowska

Malgorzata Kęsik-Brodacka

Andrzej Płucienniczak

Natalia Lukasiewicz

Beata Gromadzka

Grażyna Płucienniczak

Diana Mikiewicz

Anna Porębska

Agnieszka Sobolewska

Józef Kapusta

Michal Odrowąż-Sypniewski

Anna Bierczynska-Krzysik

Anna Bierczyńska-Krzysik

Krzysztof Kucharczyk

Agnieszka Romanik

Monika Olszewska

Agnieszka Sirko

Anna Góra-Sochacka

Włodzimierz Zagórski-Ostoja

Grażyna Plucienniczak

Anna Stachyra

Agnieszka Romanik-Chruścielewska

Róza Sawicka

Bogusław Szewczyk

Violetta Saczynska

Anna Porebska

Agnieszka Romanik-Chruscielewska

Grazyna Plucienniczak

Malgorzata Kesik-Brodacka

Krzysztof Smietanka

Michal Odrowaz-Sypniewski

The object of the invention are monoclonal antibodies against hemagglutinin of H5-serotype influenza viruses selected from the group comprising G-1-31-22, G-2-14-10, G-5-32-5, G-6-42-42, G-7-24-17 and G-7-27-18, having a broad application in immunoprophylaxis and immunotherapy of infections evoked by H5-serotype influenza viruses in humans and animals. The invention also provides hybridomas producing said antibodies, as well as compositions and diagnostic kits containing said antibodies for the detection and typing of H5-serotype influenza viruses and antibodies against H5-serotype influenza viruses in biological samples.

Monoclonal antibodies against hemagglutinin of h5-serotype influenza viruses and their uses, hybridomas producing said antibodies, compositions and diagnostic kits

The invention relates to an influenza viruses hemagglutinin protein as a vaccine antigen against influenza viruses, in particular to a protein being a fragment of H5 hemagglutinin from highly pathogenic avian influenza virus (HPAIV) H5N1 strain, expressed in a bacterial expression system. The protein according to the invention is a potential ingredient of an anti-HPAIV H5N1 vaccine, administered to chickens parenterally or through mucous membranes in the presence of adjuvants. The object of the invention is also a method of obtaining the vaccine protein.

Influenza virus hemagglutinin protein as a vaccine antigen

The object of the invention is a DNA vaccine, method of inducing the immune response, antibodies specifically recognizing the haemagglutinin H5 of an influenza virus and application of the DNA vaccine. According to the invention, one or two-fold immunization of hens with DNA vaccine containing a cDNA encoding the modified H5 haemagglutinin HA protein, i.e. with the deletion of the cleavage site between HA subunits (this provides for greater safety of the vaccines). Moreover, the encoding region of the HA is modified in such a way that protein production in the bird cells should achieve maximal yield. The main modification is codon optimization for the hens and deletion of the site of proteolytic cleavage between subunits HA1 and HA2.

DNA vaccine, method of inducing the immune response, method of immunisation, antibodies specifically recognising the H5 haemagglutinin of an influenza virus and use of the DNA vaccine

Growing community of inventors

Iwaniska, Poland

Katarzyna Florys