Rutgers, The State University of New Jersey

Senesco, Inc.

University of Medicine and Dentistry of New Jersey

Katalin A Hudak

John E Thompson

Tzann-Wei Wang

Nilgun Ereken Tumer

Yuwen Hong

Jonathan D Dinman

Bijal Parikh

Disclosed are PAP mutants that are less toxic than wild type PAP and that exhibit depurination activity. Also disclosed are transgenic plants that procedure the PAP mutants, and methods for preparing the plants. Further disclosed are bioconjugates containing the PAP mutants, pharmaceutical compositions containing the bioconjugates, and methods of administering the compositions to treat disease.

C-terminally truncated non-cytotoxic PAP mutants

Disclosed are transgenic plants containing an exogenous nucleic acid encoding an L3 protein. The plant exhibits increased resistance to viruses and/or fungi that infect plants. The L3 proteins include wild-type proteins, spontaneously occurring mutants and non-naturally occurring L3 mutants. Also disclosed are methods of reducing the toxicity of single-chain ribosome inhibitory proteins in cells, e.g., yeast, plant and animal cells, by co-administering the L3 protein with the RIP. Further disclosed are non-naturally occurring L3 mutants that (a) substantially fail to bind single-chain RIPs that bind endogenous L3 proteins, (b) are unable to maintain M1 killer virus, (c) promote altered programmed ribosomal frameshift efficiency, (d) exhibit resistance to peptidyltransferase inhibitors, and combinations of any of (a)&#x2013;(d).

Virus-resistant transgenic plants expressing L3

Regulation of expression of senescence in plants is achieved by integration of a gene or gene fragment encoding senescence-induced lipase into the plant genome in antisense orientation. The carnation andgenes encoding senescence-induced lipase are identified and the nucleotide sequences are used to modify senescence in transgenic plants.

DNA encoding a plant lipase, transgenic plants and a method for controlling senescence in plants

Regulation of expression of senescence in plants is achieved by integration of a gene or gene fragment encoding senescence-induced lipase into the plant genome in antisense orientation. The carnation and Arabidopsis genes encoding senescence-induced lipase are identified and the nucleotide sequences are used to modify senescence in transgenic plants.

Growing community of inventors

East Brunswick, NJ, United States of America

Katalin A Hudak