Genentech, Inc.

F. Hoffmann-la Roche Ag

Karthik Veeravalli

Michael W Laird

Michael Patrick Dillon

Christoph Spiess

Twyla Noelle Lombana

Rebekah McKenna

Rebekah Mckenna

Provided herein are methods and host cells for producing a polypeptide containing two chains, such as an antibody, half-antibody, antibody fragment, or one-armed antibody. The methods and host cells allow for two-chain polypeptide production using expression of polynucleotides encoding the polypeptide chains from extra-chromosomal polynucleotide(s), and expression of one or more chaperone protein(s) (e.g., peptidyl-prolyl isomerases and/or protein disulfide oxidoreductases) from the host cell chromosome using non-native combination(s) of promoters and translational units encoding a chaperone protein.

Methods of producing two chain proteins in prokaryotic host cells

The invention provides methods of identifying bacteria comprising binding polypeptides. The invention also provides methods of identifying bacteria with improved expression of binding polypeptides. The invention also provides methods of identifying binding polypeptides with improved expression. The invention also provides engineered bacteria suitable for use in the methods of the invention. The invention also provides compositions that can be obtained using the methods, for example, anti-interleukin-13 (IL-13) antibodies with improved expression and/or stability. The invention also provides libraries comprising binding polypeptide (e.g., antibody) variants.

Methods of identifying bacteria comprising binding polypeptides

The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.

Microorganisms comprising a mutant metA allele for reducing norleucine misincorporation into proteins

Methods for reducing norleucine misincorporation into proteins using a microorganism comprising a mutant metA allele

Compositions for preventing norleucine misincorporation into proteins

Methods and compositions for preventing norleucine misincorporation into proteins

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San Francisco, CA, United States of America

Karthik Veeravalli