Deutsches Krebsforschungszentrum Stiftung Des Offentlichen Rechts

Deutsches Krebsforschungszentrum Stiftung Des Oeffentlichen Rechts

Karsten Richter

Ansgar Haking

Michael Trendelenburg

Manfred Wiessler

Stefan Raddatz

Helmut Tr&ouml;ster

Eberhard Spiess

Helmut Troester

Sabine Bub

The invention relates to plasmids derived from pBluescript KS(&plus;), which contains more than 1, preferably 2, 7, 14, 21 and 27, respective SK primer elements, and their use in analytical electron microscopy.

Molecular-biological marker for analytical electron microscopy

A method for detecting an element in a sample using a transmission electron microscope to measure a first image of the intensities of the sample at an energy loss in the range of the element signal. The background at various points is determined in a comparator sample which does not contain the element intensity. Energy loss is determined at the front of the element signal, and an energy loss is determined in the range of the element signal. From these values an approximation function is calculated so that an image of the pure element-specific intensities can be calculated.

Method for detecting an element in a sample

The intention is to provide a process for the contrast enhancement of a specific particle in an image of a specimen, taken by a transmission electron microscope, in which a calculated contrast-rich image is created by way of the background intensities calculated from the intensities of a first image being drawn off pixel by pixel, whereby the background intensities are calculated as a function of the intensities of a second image. In this situation, it is intended that this process should feature a higher range of application than processes known hitherto, and, in particular, are well-suited for contrast enhancement for gold particles in immuno-gold marking. It is proposed, for this purpose, that the first image be taken under conditions in which the particle features the highest possible contrast, and that the second image is taken in a selected energy window which is selected in such a way that the contrast difference between the two images for the particle differs by the corresponding contrast difference for at least a second specimen constituent.

Method for enhancing the contrast for a transmission electron microscope

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Ketsch, Germany

Karsten Richter