Institute for Drug Research Ltd.

Biogal Gyogyszergyar Rt.

Richter Gedeon Vegyeszeti Gyar Rt

Teva Gyogyszergyar Zartkoruen Mukodo Reszvenytarsasag

Biogal Gyogyszergyar

Karoly Albrecht

Istvan M Szabo

Istvan Barta

Julianna Mozes Nee Suto

Ildiko Lang

Gabor Ambrus

Attila Andor

Janos Erdei

Eva Ilkoy

Valeria Szell

Gyula Horvath

Antonia Jekkel

Gyorgy Somogyi

Margit Bidlo Nee Igloy

Eva Gulyas

Gabor Balogh

Sandor Boros

Aniko Tedges

Janso Salat

Magdolna Petroczki

Attila Konya

Imre Moravcsik

Antonia Jekkel Nee Bokany

Kalman Konczol

Laszlo Birincsik

Tibor Lang

Gyorgy Santha

Eva Toth-Sarudy

Kalman Polya

Bela Makadi

Emilia Simonovits

Eva Tomori Nee Joszt

Lajos Kiss

Karoly Nagy

Gabor Hantos

Istvan Mihaly

Agota Hulber Nee Dobos

Laszlo Birinesik

Bela Palotas

Karoly Buzasi

Etelka Deli Nee Konszky

Aniko Molnar Nee Antal

Vilma Szaszhegyesi

Zsuzsanna Lengyel Nee Szemenyei

Eva Csajagi

Zsuzesanna Vida

Janos Salat

A procedure for the preparation of pseudomonic acid A comprising submerged cultivation of abacterium strain capable of biosynthesis of the substantially pure pseudomonic acid A in aerated conditions via fermentation; and isolation of the desired compound is disclosed. In particular, the procedure of the present invention comprises cultivation of thesp. bacterium strain No. 19/26 deposited under accession No. NCAIM(P)B 001235 in the National Collection of the Agricultural and Industrial Microorganisms, Budapest, Hungary, or its pseudomonic acid A-producing mutant or variant, on a medium at a temperature of between about 20&#xb0; C. and 30&#xb0; C. containing organic nitrogen and carbon sources and, optionally, mineral salts.

Process for the preparation of pseudomonic acid a antibiotic by microbiological method

Microbial process for preparing pravastatin

A procedure for the preparation of pseudomonic acid A comprising submerged cultivation of a Pseudomonas bacterium strain capable of biosynthesis of the substantially pure pseudomonic acid A in aerated conditions via fermentation; and isolation of the desired compound is disclosed. In particular, the procedure of the present invention comprises cultivation of the Pseudomonas sp. bacterium strain No. 19/26 deposited under accession No. NCAIM(P)B 001235 in the National Collection of the Agricultural and Industrial Microorganisms, Budapest, Hungary, or its pseudomonic acid A-producing mutant or variant, on a medium at a temperature of between about 20&deg; C. and 30&deg; C. containing organic nitrogen and carbon sources and, optionally, mineral salts.

Process for the preparation of pseudomonic acid A antibiotic by microbiological method

The invention relates to a microbial process for the production of immunosuppressive cyclosporine antibiotic complex or of its components, cyclosporine A, cyclosporine B and cyclosporine C, by the aerobic fermentation of a filamentous fungus strain biosynthesizing the above antibiotic(s) in a nutrient medium containing utilizable carbon and nitrogen sources as well as mineral salts, and by isolating the products formed, which comprises culturing a strain of the novel Tolypocladium varium fungus species producing the cyclosporine antibiotic complex, preferably tolypocladium varium sp. nov. CY/93, deposited at the National Collection of Agricultural and Industrial Microorganisms, Budapest, Hungary under the number NCAIM(P)F 001005, on a nutrient medium containing carbon sources, organic and inorganic nitrogen sources as well as mineral salts, under aerobic conditions, at 25.degree. to 30.degree. C., and, if desired, isolating and purifying the cyclosporine antibiotic complex or its components produced.

Microbial process for the production of immunosuppressive antibiotics

The invention relates to a process for the production of 9.alpha.-hydroxy-4-androstene-3,17-dione from natural sterols of plant or animal origin or from mixtures thereof by the submerged aerobic fermentation of an enzyme-deficient, sterol-de-grading microorganism in a nutrient medium containing utilizable carbon and nitrogen sources as well as mineral salts, and by isolating the product formed, which comprises transforming the sterol or sterol mixture with the culture of a 1,2-steroid-dehydrogenase deficient strain of new Mycobacterium roseum species which is able to degrade the side-chain of natural sterols, preferably with Mycobacterium roseum sp. nov. 1108/1 deposited at the National Collection of Agricultural and Industrial Microorganisms, Budapest, Hungary under the number NCAIM B (P) 000339, and by isolating and purifying the 9.alpha.-hydroxy-4-androstene-3,17-dione obtained.

Microbiological process for preparing

Growing community of inventors

Budapest, Hungary

Karoly Albrecht