Agency for Science, Technology and Research

Karl Schumacher

Jackie Yi-Ru Ying

Karthikeyan Narayanan

Hanry Yu

Motoichi Kurisawa

Rensheng Deng

Andrew Chwee Aun Wan

Min Hu

Shyi-Herng Kan

Wanxin Sun

Gunter Maubach

Annegret Schumacher

Shyi Herng Kan

Susanne San San Ng

Jeremy Loh Ming Hock

James Tseng-Ming Hsieh

Jackie Y Ying

A polymer fiber is formed by hydrodynamic spinning. Fluids are forced to flow through a conduit to form a laminar flow comprising three or more layers of generally coaxial fluid flows, at respective flow rates selected to define a cross-section of a tubular middle layer of the fluid flows. The middle layer comprises a cross-linkable polymer precursor. Another layer of the fluid flows comprise a cross-linking agent. The polymer precursor, cross-linking agent and fluids are selected to prevent substantial diffusion of the polymer precursor away from the middle layer, and to allow a portion of the cross-linking agent to diffuse from the another layer into the middle layer to facilitate cross-linking of the polymer precursor in the middle layer to form a tubular polymer layer in a polymer fiber. The polymer layer thus has a cross-section generally corresponding to the cross-section of the middle layer.

Hydrodynamic spinning of polymer fiber in coaxial laminar flows

The present invention relates to methods of differentiating a human embryonic stem (ES) cell into a cell, specifically a renal epithelial cell, expressing AQP-I. The methods disclosed comprise culturing human ES cells in a renal specific medium in the presence of an extracellular matrix molecule. The cells produced according to said method can be used to treat renal related disorders such as renal failure, nephrosis, Bright's disease and glomerulitis.

Method for differentiating embryonic stem cells into cells expressing AQP-1

There is provided a method of culturing a stem cell on extracellular matrix extracted from support cells and in a stem cell culture medium comprising medium conditioned by the support cells.

Method for extracellular matrix mediated differentiation and proliferation of stem cells

The present invention relates to articles and methods involving porous materials (e.g., membranes) which may interact with species, such as biological molecules, cells, etc., whereby the species may adhere to or become immobilized with respect to a surface of the porous material or an adhesion layer coating the porous surface. The porous material may be capable of attaching species with control over the positioning and spatial distribution of the species across the surface of the material. Such articles and methods may be useful in, for example, biological assays, biological sensors, or in the culturing of biological cells.

High throughput cell-based assays fabricated with integrated silicon and cell culture technologies

Methods and apparatuses involving biocompatible structures for tissue engineering and organ replacement and, more specifically, biocompatible structures formed by three-dimensional fabrication, are described. In some embodiments, the biocompatible structures are scaffolds for cells that can be used as tissue engineering templates and/or as artificial organs. The structures may be three-dimensional and can mimic the shapes and dimensions of tissues and/or organs, including the microarchitecture and porosities of the tissues and organs. Pores in the structure may allow delivery of molecules across the structure, and may facilitate cell migration and/or generation of connective tissue between the structure and its host environment. Structures of the invention can be implanted into a mammal and/or may be used ex vivo as bioartificial assist devices.

Three-dimensional fabrication of biocompatible structures in anatomical shapes and dimensions for tissue engineering and organ replacement

Growing community of inventors

Singapore, Singapore

Karl Schumacher