Applied Biosystems, LLC

Applera Corporation

Proteinsimple

Cell Biosciences, Inc.

Karl O Voss

Aldrich N K Lau

Roger A O'Neill

Tom Weisan Yang

Marc Glazer

Andrei Bordunov

Arunashree Bhamidipati

Daniel J Suich

Kevin J Hacker

James Eugene Knittle

The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided.

High speed, high resolution compositions, methods and kits for capillary electrophoresis

Methods for detecting one or more analytes, such as a protein, in a fluid path are provided. The methods include resolving, immobilizing and detecting one or more analytes in a fluid path, such as a capillary. Also included are devices and kits for performing such assays.

Methods and devices for analyte detection

Methods and apparatus are provided to resolve analytes within a fluid path using isoelectric focusing, gel electrophoresis, or other separation means. Materials within the fluid path that are compatible with these separation means are used to attach resolved analytes to the wall of the fluid path. Attachment results from a triggerable event such as photoactivation, thermal activation, or chemical activation. In accordance with a further aspect of the present invention, the material in the capillary may also be disrupted, by either the triggerable event or a subsequent event such as melting or photocleavage. Thus, an open lumen or porous structure may be created within the fluid path, allowing unbound analyte materials to be washed from the fluid path, and detection agents to be washed into the fluid path. The separation-compatible materials may be polymerizable monomers, gels, entangled polymers or other materials.

High speed, high resolution compositions, methods, and kits for capillary electrophoresis

Various embodiments provide, for example, buffer compositions and/or sieving formulations useful in connection with electrophoresis instruments, such as capillary electrophoresis (CE) devices. In various embodiments, a buffer composition can include Bis-Tris, TAPS and/or TAPSO, and, optionally, a chelating agent, such as EDTA. Methods of separating samples containing bio-molecules, such as DNA or RNA, are also described.

Buffers for electrophoresis and use thereof

Methods and devices are described for concentration and cleanup of samples containing bio-molecule analytes (e.g., polynucleotides, such as DNA, RNA, PNA). Various embodiments provide for pH-mediated sample concentration and cleanup of nucleic acid samples with channel devices (e.g., cross-T format, microchannel devices).

Concentration and cleanup of nucleic acid samples

Methods for detecting one or more analytes, such as a protein, in a fluid path are described herein. In some embodiments a method includes resolving one or more analytes in a fluid path, such as, for example, a capillary. After the one or more analytes are resolved, the one or more analytes are bound to the fluid path upon activation of one or more triggerable agents disposed within the fluid path. The one or more analytes that are bound to the fluid path are detected within the fluid path.

Compositions and methods of use are disclosed for the analysis of polynucleotides isolated in individual reaction compartments.

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Foster City, CA, United States of America

Karl O Voss