Boehringer Mannheim Gmbh

Roche Diagnostics Gmbh

Karl-Heinz Schleifer

Anne Stern

Hans Seidel

Albert Roder

Hans Mollering

Friedrich Gotz

Erich Elstner

Christoph Kessler

Ruediger Rueger

Wolfgang Ludwig

Barbara Sedewitz

For the genus-specific or/and species-specific detection of bacteria in a sample liquid, bacterial RNA is hybridized with a primer which is complementary to a genus-specific or species-specific region of the RNA of particular bacteria or to a highly conserved region of the RNA of bacteria in general, but which is not complementary at its 3&prime; end to the RNA of bacteria of another genus or species, the primer is elongated in the presence of a suitable polymerase and the four deoxyribonucleotides, if desired, with a concurrent or subsequent labelling of the elongation product and an elongation product formed is hybridized with a genus-specific or species-specific oligonucleotide after denaturation and the hybridization is detected by means of the oligonucleotide label.

Method for the genus-specific or/and species-specific detection of bacteria in a sample liquid

The present invention provides a new pyruvate oxidase which decarboxylates pyruvate with the formation of hydrogen peroxide, characterized in that it is active without the addition of FAD, TPP and divalent metal ions. The present invention also provides a process for preparing this new enzyme and a reagent containing it.

Pyruvate oxidase

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Unterschleissheim, Germany

Karl-Heinz Schleifer