Atto-Tec Gmbh

Other

Boehringer Mannheim Gmbh

Evotec Ag

Karl-Heinz Drexhage

Jutta Arden-Jacob

Norbert Kemnitzer

Alexander Zilles

Monika Hamers-Schneider

Hans-Peter Josel

Rupert Herrmann

Joachim Fries

J&uuml;rgen Wolfrum

Eloisa Lopez-Calle

J&ouml;rg Frantzeskos

Elisabeth Drexhage

Norbert Uwe Kemnitzer

The invention relates to compounds of the general formulae (I)-(IV), which are characterized by substituents B comprising one or more sulfonic acid groups and their use as marker groups for the detection of analytes.

Polysulfonated fluorescence dyes

The present invention relates to polycyclic compounds of the general formula (I) which are distinguished by an amine substituent NRRon the central carbon atom of the chromophore, to processes for their preparation, and to the use thereof for the determination of analytes.

Amine-substituted tricyclic fluorescent dyes

The invention concerns the production of quinoline compounds containing sulfonic acid groups, the said quinoline compounds and their conversion into dyes containing sulfonic acid groups. The dyes according to the invention are used especially to label analytes, for example to label biomolecules.

Sulfonamide derivatives of polycyclic dyes used for analytical applications

The present invention relates to carboxamide-substituted dyes, the production and use of such dyes as labeling groups in analytics.

Carboxamide-substituted dyes for analytical applications

The invention relates to oxazine derivatives by means of which a broad spectrum of material to be examined can be marked and identified using fluorescence techniques.

Oxazine derivatives

The invention relates to the use of carbopyronine compounds of general formula (I) as marker groups in methods for detecting analytes. The invention also relates to novel carbopyronine compounds and to a method for producing same.

Carbopyronine fluorescent dyes

A class of asymmetric monobenzoxanthene compounds useful as fluorescent dyes are disclosed having structure (I) wherein Y and Y are individually hydroxyl, amino, imminium, or oxygen, R -R are hydrogen, fluorine, chlorine, alkyl, alkene, alkyne, sulfonate, amino, amido, nitrile, alkoxy, linking group, and combinatios thereof, and R is acetylene, alkane, alkene, cyano, substituted phenyl, and combinations thereof. The invention further includes novel intermediate compounds useful for the synthesis of asymmetric benzoxanthene compounds having general structure (II) where substituents R -R correspond to like-referenced substituents in the structure of described above, and Y is hydroxyl or amine. In another aspect, the invention includes methods for synthesizing the above dye compounds and intermediates. In yet another aspect, the present invention includes reagents labeled with the asymmetric benzoxanthene dye compounds, including deoxynucleotides, dideoxynucleotides, phosphoramidites, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.

Amidopyrylium fluorescence dyes

In order to carry out optical detection and identification of individual tumor markers in undiluted blood plasma, said tumor markers are marked with fluorescent dyes which are characterized by specific fluorescent lifetimes ranging from 0.5 to 6. The blood plasma is exposed to pulsed diode laser radiation emitted in the 630 to 670 nm wavelength range. The emission wavelengths of the dyes are selected in such a way that they are 10 to 60 nm longer than the wavelength of the diode laser. Blood plasma decay curves are detected using time-correlated single photon counts. Said curves are described by a bi-exponential model wherein it is assumed that a fixed fluorescent lifetime for blood plasma emission is 300 ps and that the marked tumor markers have a variable fluorescent lifetime. The relative amount of fluorescent photons in the marked tumors is determined in relation to the overall number of photons detected. If this value exceeds 0.3, a tumor marker is present in the observed volume. The tumor markers can also be identified by the lifetimes which are determined through bi-exponential evaluation. In this manner, individual tumor markers can be detected and identified in undiluted blood plasma.

Method for optical detection of analyte molecules in a natural biological medium

Pentacyclic derivatives having the general formulae (Ia), (Ib) and (Ic) ##STR1## denote: hydrogen, alkyl with 1 to 20 carbon atoms polyoxyhydrocarbyl, phenyl, phenylalkyl with 1 to 3 carbon atoms in the alkyl chain, wherein the alkyl residues or/and phenyl residues can be substituted by one or several hydroxy, halogen, sulfo, carboxy or alkoxycarbonyl groups in which alkoxy can have 1 to 4 carbon atoms;

Pentacyclic compounds and their use as absorption or fluorescent dyes

The present invention relates to chemical substances having dye properties, or more particularly, to improved cyanine dyes and to their application in photographic bleaching processes. Preferably, a chromylium group on the hetero ring comprises either a substituted phenyl group or an alkyl group. More particularly, O-halogenated phenyl and t-butyl groups have proven to be advantageous substituents.

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Siegen, Germany

Karl-Heinz Drexhage