The University of Texas System

Karl E Klose

Bernard Arulanandam

James Chambers

Jieh-Juen Yu

M Neal Guentzel

The instant invention concernsbacteria mediated degradation of alkaline phosphatase (AP). Detection of AP degradation may be used to determine the presence ofbacteria in a sample. Furthermore, methods for identifying and treatinginfections by detecting AP degradation are described. Methods of the invention also concerns methods for treatinginfection by inhibiting AP degradation.

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Disclosed arecomprising a mutated transcriptional regulatory protein (ToxT) amino acid sequence, wherein the mutation results in a reduction in the expression of cholera toxin by the. Also disclosed arecomprising a mutated transcriptional regulatory protein (ToxT) amino acid sequence, wherein the mutation results in an increase in transcription of the nucleic acid encoding the mutated ToxT. Vaccines comprising the aforementionedare disclosed. Also disclosed are amino acid sequences that encode a mutated ToxT protein.

Identification of residues critical for the function of the vibrio cholerae virulence regulator ToxT by scanning alanine mutagenesis

In one embodiment, there is disclosed a method of inducing an immune response in a subject comprising administering to the subject abacterium that includes an alteration in the nucleic acid sequence encoding the mglA, iglA, iglB, iglC, or iglD gene of the bacterium.

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San Antonio, TX, United States of America

Karl E Klose