Monsanto Technology LLC

Karina Krasomil-Osterfeld

Barry S Goldman

Thomas M Malvar

Wei Wu

John W Pitkin

Steven C Slater

Jiamin Zeng

Gregory Hinkle

David Richard Corbin

James Arthur Baum

James Kevin Roberts

William P Donovan

Judith C Donovan

Joseph E Huesing

James T Engleman

Sergei Spiridonov

The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space fromand related strains. The proteins are isolated from culture supernatants ofand related strains and display insecticidal activity against coleopteran insects including Colorado potato beetle () and Southern Corn Rootworm (). Insecticidal proteins encoded by nucleotide sequences that hybridize to the isolated and characterized nucleotide sequences are disclosed. Also disclosed are methods of making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.

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The present invention relates to a nucleic acid sequences fromthat encodes a GroEL protein. The invention encompasses nucleic acid molecules present in non-coding regions as well as nucleic acid molecules that encode proteins, fragments of proteins, tRNA's, fragments of tRNA's, rRNA's and fragments of rRNA's. In addition, protein and fragments of proteins so encoded and antibodies capable of binding the proteins are encompassed by the present invention. The invention also relates to methods of using the disclosed nucleic acid molecules, proteins, fragments of proteins, RNA'S, and antibodies, for example, for gene identification and analysis, and preparation of constructs.

The invention provides isolated nucleotide sequences fromspecies Xs86068, and, in particular, nucleotide sequences that encode insect inhibitory proteins, the insecticidal proteins, and compositions that comprise one or more of the insecticidal proteins for use in controlling insect infestation.

The present invention relates to nucleic acid sequences from, and, in particular, to genomic DNA sequences, and to nucleotide sequences that encode insecticidal proteins from, strain Xs85831. The invention encompasses nucleic acid molecules present in non-coding regions as well as nucleic acid molecules that encode proteins, fragments of proteins, tRNA's, fragments of tRNA's, rRNA's, and fragments of rRNA's. In addition, proteins and fragments thereof and antibodies capable of binding specifically to the proteins are encompassed by the present invention. The invention also relates to methods of using the disclosed nucleic acid molecules, proteins, protein fragments, RNA's, and antibodies, for example, for gene identification and analysis, preparation of constructs, and protecting plants from insect infestations.

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Ellisville, MO, United States of America

Karina Krasomil-Osterfeld