Alligator Bioscience Ab

Karin Haraldsson

Christina Furebring

Peter Ellmark

Erika Gustafsson

Björn Walse

Ann-Christin Malmborg Hager

Anna Rosén

Johannes A G Van Strijp

Cornelis P M Van Kessel

Fredrik Karlsson

Petrus J A Haas

Lena Schultz

Marie Karlsson

The present invention provides a polypeptide having a biological activity of the Chemotaxis Inhibitory Protein of('CHIPS'), the polypeptide comprising or consisting of the amino acid sequence of SEQ ID NO: 2, or a fragment or variant thereof having a biological activity of CHIPS, wherein the fragment or variant retains amino acid substitutions K40E, D42V, N77H, K100R, K105R, N111 K and/or G112A relative to the wildtype CHIPS protein of SEQ ID NO:1. In one embodiment, polypeptide consists of the amino acid sequence of SEQ ID NO: 2. Related aspects of the invention provide pharmaceutical compositions comprising a polypeptide of the invention, together with methods or making and using the same.

Polypeptides and use thereof

The present invention provides a polypeptide having a biological activity of the Chemotaxis Inhibitory Protein of('CHIPS'), the polypeptide comprising a variant of the amino acid sequence of SEQ ID NO:1. Preferably, the polypeptide is a CHIPS variant wherein one or more of the following amino acids is modified: N31, S32, G33, L34, P35, K40, D42, R46, Y48, K50, G52, T53, K54, N55, S56, A57, Q58, K61, E67, K69, L76, N77, P79, D83, L90, K92, K100, K101, S104, K105, S107, Y108, N111 and G112. In a preferred embodiment, the polypeptide is less immunogenic hi humans than the wildtype CHIPS protein. The invention further provides methods of making and using such variant CHIPS polypeptides.

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Lund, Sweden

Karin Haraldsson