Valtion Teknillinen Tutkimuskeskus

Nokia Corporation

Honeywell Oy

Kari Kataja

Hans Söderlund

Kristiina Takkinen

Reetta Satokari

Marja T Paloheimo

Jakke Mäkelä

Marja Ilmen

Jouko Malinen

Reijo Kuusela

Ari Hokkanen

Janne Aikio

Ingmar Stuns

Kai Kolari

Jan Rautio

Teemu Alajoki

Sanna Aikio

Hannu Vasama

Sini Kivi

Stella Rovio

Hans Soederlund

The invention is related to a method and test kits for quantitative determination of polynucleotide amounts present in a sample. The test kit comprises organized pools with polynucleotide probes having distinct sizes and optionally provided with tracer tags or primer tags. The probes are allowed to hybridize with affinity tagged analyte polynucleotides from the sample. The result is hybrids, which can be recovered on a separation aiding tool provided with the pair of the affinity tag. After the quantitative release of the probes, the probes are either directly recorded, or if primer tagged, they are amplified and optionally provided with a tracer tag before recording. The invention provides a sensitive and quantitative determination of the amount polynucleotides present in a cell or tissue sample and allows a quantitative assessment of variations in the amounts of polynucleotides as a response to inherent changes or due to external stimuli.

Method for determining amounts of polynucleotide sequences present in cell or tissue samples

A microfluidic chip device (MCD) and its use for performing miniaturized assays on magnetic microbeads (MMs) are described. The MCD is particularly useful for carrying out miniaturized transcript analysis by aiding affinity capturing (TRAC) assays, including PCR. The MCD comprises at least one reaction chamber with sealable liquid connections and at least one fluidic pillar filter in each chamber. The fluidic pillar filter comprises rods with spacings allowing MMs to pass. The sealable liquid connections feed liquid to the reaction chamber, wherein air bubbles are removed. The liquid stream contacts the MMs, which are manipulated with a magnetic rod. The liquid connections enable trapping of the MMs behind the pillar filters or in the channel, while the liquid is changed.

Microfluidic chip devices and their use

An optical or infrared spectrometer is suitable for on-line measurements for industrial, agricultural, field, commercial and other applications. Optical spectrometers are very useful for various analytical measurements. On-line operation is needed for obtaining real-time information, which is useful e.g. for process automation and quality control needs. The invention is based on optical design optimized for measuring moving samples at a distance and includes a light guide for signal homogenization, a linear variable filter for defining multiple measurement wavelengths as well as a linear detector array for detecting optical signals relating to the different wavelengths. There is an element for cooling and stabilizing the operating temperature of both the linear detector array and the linear variable filter, while the spectrometer is operating in variable environmental conditions. Thanks to the optical signal chain designed to maximize the radiance at the detector, the proposed spectrometer can provide high signal-to-noise ratio and high speed.

Spectrometer for measuring moving sample material and the method

The invention relates to a method and test kit for quantitative determination of the amounts or relative proportions of polynucleotides in a mixture. The invention enables assessment of dynamic variations in a mixed population of organisms using affinity aided solution hybridization. The test kit comprises organized pools of polynucleotide probes having approximately the same number of nucleotides, which are distinguishable using resolution enabling tags providing the probes with different sizes. The resolution enabling tags may simultaneously act as tracer, affinity or primer tags. The probes are allowed to hybridize with affinity tagged analyte polynucleotides. The result is hybrids, recoverable on separation aiding tools provided with counterparts of the affinity tag. After the quantitative release of the probes, the individual probes can be amplified and recorded. The method and test kit are useful for determining hygienic and epidemiologic situations and evaluating the effect of antibiotic treatment and sanitary measures.

Method and test kit for quantitative determination of polynucleotides in a mixture

A method and miniaturizable device is described to read/write information to an optical storage medium (). A device comprises one or more light sources () bounded up with an access unit () which is arranged to be controllable to a position, in which light beams () are transmitted transversal towards an optical storage medium, and reflected light beams () are analysed by a detector element () which further informs the access unit whether to move or stand still to keep light beams in focus and on track. A device according to the invention is possible to implement in a small size and low weight due to reduced component count and thin access unit geometries. A communication device () according to the invention may be implemented to fulfil a crucial need for ultraminiature range of communication devices.

Read write device for optical memory and method therefore

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Espoo, Finland

Kari Kataja