Cambridge Bioscience Corporation

Karen E DeBell

John W Roberts

A tetrazolium-based system for testing for the presence of organisms or cells in a specimen includes in one embodiment a mixture of a substrate and an indicator system, wherein the overall system is buffered to a pH above 8.0, and in a preferred embodiment at about a pH of 9.0 when mixed. The substrate solution and indicator solution are not mixed until shortly before use. The indicator solution is buffered at a pH of approximately 3.3, thereby enhancing the shelf life. The substrate may consist of glucose with at least one amino acid in a buffered solution formulated such that, by mixing equal parts with the indicator solution, a pH above 8, preferably 9.0 results.

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Easton, MA, United States of America

Karen E DeBell