University of California

Karen A McDonald

Bryce William Falk

Abhaya M Dandekar

Mysore R Sudarshana

Benjamin E Lindenmuth

Sandra L Uratsu

Nathaniel J Kingsbury

Michael A Plesha

Ting-Kuo Huang

Minsook Hwang

Carroll E Cross

Sang-Kyu Jung

Kalimuthu Karuppanan

Jason P Eiserich

Alan P Jackman

Melody M Trexler

The present invention provides plant-made elafin-Fc fusion proteins for treating inflammatory diseases, e.g., inflammatory lung diseases. In certain embodiments, the fusion proteins comprise one or more point mutations, which confer improved properties, such as increased resistance to oxidation, cleavage, and increased half-life. The present invention additionally provides polynucleotides encoding the fusion proteins, recombinant cells and expression vectors, and transgenic plants comprising the fusion protein coding sequences. The present invention further provides methods for the production of the fusion proteins.

Fusion proteins for treating inflammatory diseases

The invention relates to a novel chemically inducible plant viral amplicon (CMViva) expression system that permits controllable, high level expression of foreign genes in plant hosts. This system employs agro-infiltration of plants to provide a transient production of a protein of interest, such as a human blood protein. This system provides a major advantage over existing plant expression systems because it allows for consistent expression of foreign or heterologous proteins in plant hosts.

Chemically inducible cucumber mosaic virus protein expression system

The present disclosure relates to methods of increasing the extraction of an apoplast-targeted recombinant protein in a plant tissue and to methods of increasing the purity of an apoplast-targeted recombinant protein recovered from a plant tissue. The methods involve contacting plant tissue with a rinse fluid on a plurality of occasions to release recombinant protein from the apoplast into the rinse fluid to create an apoplast wash fluid. The resulting content of the recombinant protein in the apoplast wash fluid is higher than the content of the recombinant protein in the apoplast wash fluid from comparable plant tissue contacted with rinse fluid on a single occasion.

Apoplast wash fluid recovery for improved recombinant endoglucanase extraction in tabacco leaves

Described herein are viral amplicon-based protein expression systems and methods useful for producing heterologous proteins, such as enzymes, by agroinfiltration. The methods involve producing anwith a Ti plasmid encoding a heterologous protein, infecting plant cells with the, allowing expression of the heterologous protein, and recovering the heterologous protein from the plant cells. In one embodiment, the protein produced is an endoglucanase.

Plant-based production of heterologous proteins

Described herein are methods useful for producing proteins, such as enzymes, by agrofiltration. The methods involve producing anwith a Ti plasmid encoding a cellulase, infecting plant cells with the, allowing expression of the cellulase, and recovering the cellulase from the plant cells. In one embodiment, the protein produced is an endoglucanase.

Production of cellulase enzymes in plant hosts using transient agroinfiltration

The present invention relates to methods for semi-continuous culture of plant cells in a nutrient medium. The pH of the medium is monitored during the cell culture as a way of monitoring the expression of a product of interest by the cells.

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Fairfield, CA, United States of America

Karen A McDonald