Ustav Makromolekularni Chemie Av Cr, V.v.i.

Ustav Organicke Chemie a Biochemie Av Cr, V.v.i.

Zentiva K.s.

Univerzita Karlova V Praze, Prirodovedecka Fakulta

Univerzita Karlova

Mikrobiologicky Ustav Av Cr, V.v.i.

Ustav Organicke Chemie a Biochemie Akademie Ved Ceske Republiky, V.v.i.

Karel Ulbrich

Blanka Rihova

Tomas Etrych

Jiri Strohalm

Jan Konvalinka

Pavel Sacha

Jiri Schimer

Vladimir Subr

Tomas Knedlik

Petr Chytil

Pavel Majer

Petr Cigler

Vaclav Navratil

Marketa Jelinkova

Frantisek Sedlak

Marek Kovar

Jan Tykvart

Tomas Mrkvan

Michal Pechar

Jirí Schimer

Ondrej Hovorka

Martin Studenovsky

Tomás Etrych

Markéta Jelinkova

A macromolecular water-soluble conjugates based on synthetic copolymers to which at least one affinity tag, at least one imaging probe and at least one targeting ligand are bound via covalent bonds. The macromolecular conjugate may be used in identification, visualization, quantification or isolation of proteins and/or cells.

Macromolecular conjugates for visualization and separation of proteins and cells

Synthetic macromolecular conjugate for selective interaction with proteins has a synthetic copolymer, and at least one binding group and at least one further group selected from an affinity tag and an imaging probe, and at least one binding group and at least one further group being bound via covalent bond to the synthetic copolymer. The macromolecular conjugate is suitable in particular for identification, visualization, quantification or isolation of proteins and/or cells.

Macromolecular conjugates for isolation, immobilization and visualization of proteins

The present invention relates to water-soluble high-molecular-weight polymer drug carriers and their conjugates with drugs, derived from dendrimers of the amidoamine and 2,2-bis(hydroxymethyl)propanoic types, the amino and hydroxy end groups of which are attached to semitelechelic copolymers of N-(2 hydroxypropyl)methacrylamide (HPMA) through biodegradable spacers. The polymer carriers and conjugates enable targeted transport notably of anticancer drugs into solid tumors in which biodegradation, the associated controlled drug release and subsequent elimination of polymer carrier from the organism are provided. The polymer carrier conjugated with a cancerostatic for use in targeted therapy of human tumors.

Dendritic high-molecular-weight polymer drug carriers and their conjugates with drugs especially for treatment of solid tumours

A polymeric drug, in which a cancerostatic connected via spacers containing hydrolytically cleavable hydrazone bonds is bound to a water-soluble polymeric carrier prepared on the basis of a N-(2-hydroxypropyl)methacrylamide copolymer, wherein the structure of the polymeric drug consists of the main chain of N-(2-hydroxypropyl)methacrylamide carrying the cancerostatic and another chain of N-(2-hydroxypropyl)methacrylamide&#x2014;a graft, which may also carry a cancerostatic, said grafts being bound to the main chain by a bond that is stable in the body and/or by a bond cleavable in the body, especially by an oligopeptide spacer selected from the series of GlyLeuGly (SEQ ID. NO. 1), GlyPheGly (SEQ ID. NO. 2), GlyPheLeuGly (SEQ ID. NO. 3) and GlyLeuPheGly (SEQ ID. NO. 4), and a method of its preparation.

Grafted macromolecular conjugates of doxorubicin with anticancer activity and method of their preparation

Conjugates consisting of a polymeric carrier constituted by 30 to 3,000 monomer units linked to form a polymeric chain, composed of a) 60 to 99% of N-(2-hydroxypropyl)methacrylamide units, b) 1 to 25% of units of methacryloylated hydrazones of &#x3b1;-amino acids, &#x3b5;-amino acids, aromatic amino acids or oligopeptides terminated with a molecule of an anthracycline cancerostatic, c) 0.5 to 15% of units of methacryloylated &#x3b1;-amino acids, &#x3b5;-amino acids, aromatic amino acids or oligopeptides or their sodium salts.

PH-sensitive polymeric conjugates of an anthracycline cancerostatic drug for targeted therapy

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Prague, Czechia

Karel Ulbrich