Mycogen Corporation

United States of America, As Represented by the Secretary of Agriculture

Genvor Inc.

Novartis Finance Corporation

Phytogen

J. G. Boswell Company

Kanniah Rajasekaran

Thirumale S Rangan

David M Anderson

Richard L Yenofsky

John W Grula

Richard Lorne Hudspeth

Jeffrey W Cary

Jesse M Jaynes

Rajtilak Majumdar

Ronald J Sayler

Nadine B Carozzi

Thomas E Cleveland

Thomas J Jacks

Karl-Heinz Van Pee

Ronald Sayler

Douglas Rice

Richard Yenofsky

Richard Lotstein

is an opportunistic, saprophytic fungus that infects maize and other fatty acid-rich food and feed crops and produces toxic and carcinogenic secondary metabolites known as aflatoxins. In vitro studies showed a five-fold increase in antifungal activity of AGM182 (vs. tachyplesin1) against. Transgenic maize plants expressing AGM182 under maize Ubiquitin-1 promoter were produced through-mediated transformation. PCR products confirmed integration of the AGM182 gene, while RT-PCR of maize RNA confirmed the presence of AGM182 transcripts. Maize kernel screening assay using a highly aflatoxigenicstrain (AF70) showed up to 72% reduction in fungal growth in the transgenic AGM182 seeds compared to isogenic negative control seeds.

Transgenic corn with antifungal peptide AGM182 (DN:0113.18)

Transgenic corn with antifungal peptide AGM182 USDA (DN:0113.18)

A transformed cotton plant. The transformed cotton plant comprises DNA derived from a source other than cotton plants, wherein the DNA, when transformed into the cotton plants, confers a phenotype not expressed inn a parent cotton.

Transformed cotton plants

The present invention provides a method of making plants resistant to plant pathogens by transformation of host cells with a nucleic acid encoding a nonheme haloperoxidase. Transgenic plants which express a nonheme chloroperoxidase thereby resulting in enhanced resistance to phytopathogens are provided.

Transformation of plants with a chloroperoxidase gene to enhance disease resistance

A transformed cotton plant. The transformed cotton plant comprises DNA derived from a source other than cotton plants, wherein the DNA, when transformed into the cotton plants, confers a phenotype not expressed in a parent cotton.

A method for producing transformed cotton plants. The method comprising providing cotton explants, incubating the cotton explant in the presence of a vector comprising a selectable marker to produce treated explants, growing the treated explants to produce callus and selecting transformed callus.

Transformation of cotton plants

A vector for transforming cotton. The vector comprising integration sequences for integrating into the genome of cotton plants, a promoter for promoting transcription in cotton plants, a DNA sequence encoding a selectable marker and a termination signal for terminating transcription in cotton plants.

A promoter isolated from a cotton gene encoding the small subunit of ribulose bisphosphate carboxylase is described. The isolated promoter is operably linked to a coding sequence of interest to make a chimeric gene.

Cotton promoter

A somaclonal variant cotton plant. The somaclonal cotton plant is produced by a method comprising providing a cotton explant, culturing the explant in a callus growth medium supplemented with glucose as a primary carbon source until secretion of phenolic compounds has ceased and undifferentiated callus is formed from the explant, culturing the undifferentiated callus in callus growth medium supplemented with sucrose as a primary carbon source until embryogenic callus is formed from the undifferentiated callus, transferring the embryogenic callus to a plant germination medium, culturing the embryogenic callus on the plant germination medium until a plantlet is formed from the embryogenic callus, transferring the plantlets to soil, growing the plantlets to produce seeds from self pollination, collecting the seeds, planting the seeds, growing the seeds under conditions to select for a desired characteristic and collecting the plants with the desired characteristics.

Cotton somaclonal variants

A method for the regeneration of a cotton plant from somatic cells. The method comprises providing a cotton explant, culturing the explant in a callus growth medium supplemented with glucose as a primary carbon source until the secretion of phenolic compounds has ceased and undifferentiated callus is formed from the explant and culturing the undifferentiated callus in callus growth medium supplemented with sucrose as a primary carbon source until embryogenic callus is formed from the callus.

Growing community of inventors

Duarte, CA, United States of America

Kanniah Rajasekaran