Dr. Chip Biotechnology Inc.

Dr. Chip Biotechnology Incorporation

Kan-Hung Lee

Shin-Hwan Wang

Chi-Horng Bair

Yih-Weng Wang

Hsiung Hsiao

Yang-Yuan Tseng

Hsing Hsiao

Chuan-Mei Tsai

Yu-Hau Shih

Kang-Jehng Chen

This invention features a biomolecule-bound substrate that includes a support made of an organic polymer and having an unmodified surface; and a plurality of unmodified biomolecules immobilized on the unmodified surface. The organic polymer is acrylic resin, polypropylene, polystyrene, polyethylene, polyvinyl chloride, polysulfone, polycarbonate, cellulose acetate, rubber, latex, polyethylene terephthalate, acrylonitrile butadiene styrene, acrylonitrile styrene, or a combination thereof. The substrate is formed by placing the unmodified biomolecules on the unmodified surface followed by ultraviolet irradiation.

Biomolecule-bound substrates

This invention relates to a biomolecule-bound substrate made of polymeric molecules, each of which contains a reacting group; and a plurality of biomolecules, each of which contains another reacting group. One of the two reacting groups is a substitute group and the other is a leaving group; and the biomolecules are covalently bonded to the solid support via a chemical ligation reaction between the two reacting groups.

Immobilization of nucleic acids

The present invention discloses pairs of oligonucleotide primers for use in detecting the presence or absence of an enterovirus in a sample. Also disclosed are synthetic nucleotide sequences capable of specifically hybridizing to a sense strand of an enterovirus nucleic acid or a nucleic acid corresponding to the sense strand. The present invention provides a method of detecting the presence or absence of an enterovirus nucleic acid in a sample as well as a kit for detecting an enterovirus in a sample. The present invention particularly provides a method and a kit for detecting and differentiating enterovirus type 71 and/or coxsackievirus A16 in a sample.

Methods for detecting and differentiating enteroviruses and the primers and probes therefor

Growing community of inventors

Sanchong, Taiwan

Kan-Hung Lee