Johns Hopkins University

Other

Duke University

United States of America As Represented by the Administrator of Nasa

Agency for Science, Technology and Research

National University of Singapore

Guilford Pharmaceuticals, Inc.

Massachusetts Institute of Technology

Leland Stanford Junior University

Boston Scientific Scimed, Inc.

Columbia University

University of South Florida

Riken Corporation

Omeros Corporation

Institute of Materials Research and Engineering

Kam W Leong

Hai-Quan Mao

Zhong Zhao

James Perry English

Wenbin Dang

Hungnan Lo

Emilie J Siochi

David Peter Nowotnik

Bruce A Sullenger

Hanry Yu

Robert Samuel Langer

Jun Li

Nancy M Holloway

Thomas August

Herman F Staats

Soman N Abraham

Stuart A Grossman

Ashley L St John

Karina Kulangara

Jun Wang

Daria D Mochly-Rosen

Drew M Pardoll

Irina Nikolaevna Kadiyala

Hyam I Levitsky

Elizabeth A Jaffee

Rachel Bright

Corinne Bright

Andrew C A Wan

Sabah Oney

Lisa A Scott-Carnell

Lisa A Scott Carnell

Shyam S Mohapatra

Robert J Linhardt

Chuan Yang

Xu Li

Peter C Searson

Hugh A Sampson

Howard Steven Rosen

Aliasger Karimjee Salem

Xin Wang

Nai-Hong Li

John J Wozniak

Howard Levinson

Vu L Truong

Glenn J Lesser

Rosa V Azhari

Ser-Mien Chia

Wen Jie

Michael F Haller

Tung Suet Ruby Lam

Krishnendu Roy

Eric Churchill

Audrey Lazenby

Hemraj A Juwarker

Patricia D'Amore

Jennifer Gamboa Jackman

Mukesh Kumar

Bart S Hendriks

Sudhakar Kadiyala

Tadao Ohno

Seeram Ramakrishna

Chee Mun Kuan

Magnus Stougaard

Suat Hong Goh

Bernard Malavaud

Shau-Ku Huang

Birgitta Ruth Knudsen

Yi-Ping Ho

Xiping Ni

Shu Qin Liu

Weiping Li

Ren-Xi Zhuo

Tzu-Chieh Ho

Catherine S Le Visage

Kevin Y Lin

Smruthi Suryaprakash

Wensin Dang

Pam Zeitlin

Ronald Rubenstein

Scott Walsh

Jørn Erland Koch

Paul K Golumbe

Lisa S Carnell

P Jay Pasricha

Xiaoru Chen

Sissel Juul

Wen Shao

Felicie F Andersen

Paul Golumbek

Robert I Garver

Kian-Ngiap Chua

Hongzhe Li

Bao Gang Peng

Yeh-Hsing Lao

Shua-ku Huang

Subramanian Kalyanasundaram

Elizabeth R Lorden

Kyle J Miller

The present invention discloses a multifunctional DNA-templated micelle system that has a payload carrier of at least a DNA bridge and a functionalized polyethylene glycol (PEG) segment. The micelle can be used to deliver molecules, such as drugs and polynucleotides, to targeted cells for pharmaceutical uses. The PEG segment provides a functional group, such as amine, for ligand conjugation. The DNA-templated micelle of the present invention is highly controllable in size, loading efficiency and tissue targeting, and can carry multiple payloads for targeted combination strategies in cancer therapy, such as gene delivery, gene therapy, and immunotherapy.

DNA-templated micelle and uses thereof

A device for promoting healing of an injury in a living being is provided. Such device is based upon an injury covering portion, which portion comprises an electroactive polymer, such as poled polyvinylidine difluoride (PVDF) or a copolymer of PVDF. The electroactive polymer has either pyroelectric properties, piezoelectric properties, or both.

Device for healing wounds

Methods of making polycationic nanofibers by grafting cationic polymers onto electrospun neutral nanofibers and polycationic nanofibers produced by the methods are provided herein. In addition, methods of using the polycationic nanofibers to reduce inflammation, to adsorb anionic compounds such as heparin or nucleic acids, to inhibit the growth of microbes or inhibit the formation of a biofilm are also provided. The polycationic nanofibers may be in a mesh and may be included in a medical device, wound dressing, bandage, or as part of a graft.

Electrospun cationic nanofibers and methods of making and using the same

A method and device for promoting healing of an injury in a living being are provided. Such method and device are based upon an injury covering portion, which portion comprises an electroactive polymer, such as poled polyvinylidine difluoride (PVDF) or a copolymer of PVDF. The electroactive polymer has either pyroelectric properties, piezoelectric properties, or both.

Device and method for healing wounds

Provided are nanoparticles comprising heparin, chitosan, and at least one immunomodulatory agent, e.g. a cytokine. The cytokine can be selected from the group consisting of TNF, IL-12, IL-2, IL-23, IL-1&#x3b1;, IL-10, IL-18, and combinations thereof. Further provided are methods of making a nanoparticle comprising mixing a first composition comprising heparin with a second composition comprising chitosan in the presence of at least one cytokine to form a third composition. Further provided are methods of modulating an immune response comprising co-administering to a subject an antigen or vaccine with nanoparticles comprising heparin, chitosan, and at least one cytokine.

Lymph node-targeting nanoparticles

A method of manufacturing and/or using a scaffold assembly for stem cell culture and tissue engineering applications is disclosed. The scaffold at least partially mimics a native biological environment by providing biochemical, topographical, mechanical and electrical cues by using an electroactive material. The assembly includes at least one layer of substantially aligned, electrospun polymer fiber having an operative connection for individual voltage application. A method of cell tissue engineering and/or stem cell differentiation that uses the assembly seeded with a sample of cells suspended in cell culture media, incubates and applies voltage to one or more layers, and thus produces cells and/or a tissue construct. In another aspect, the invention provides a method of manufacturing the assembly including the steps of providing a first pre-electroded substrate surface; electrospinning a first substantially aligned polymer fiber layer onto the first surface; providing a second pre-electroded substrate surface; electrospinning a second substantially aligned polymer fiber layer onto the second surface; and, retaining together the layered surfaces with a clamp and/or an adhesive compound.

Aligned and electrospun piezoelectric polymer fiber assembly and scaffold

The present invention relates, in general, to agents that modulate the pharmacological activity of nucleic acid molecules and, in particular, to agents that bind therapeutic or diagnostic nucleic acid molecules in a sequence independent manner and modulate (e.g., inhibit or reverse) their activity. The invention also relates to compositions comprising such agents and to methods of using same.

Method of modulating the activity of a nucleic acid molecule

Method of treating food allergies by administering a nanoparticle comprising heparin and chitosan encapsulating IL-12

A method for protection of tissues subject to ischemic and/or reperfusion damage is provided. The method includes administering to the tissue a composition comprising nanodevices. The nanodevices can take the form of, for example, polymeric nanoparticles or lipidic nanoparticles. The nanodevices also find use in methods for reducing ischemic injury in tissue at risk of such injury, such as heart and brain tissue.

Method and use of nano-scale devices for reduction of tissue injury in ischemic and reperfusion injury

Methods for the identification of microorganisms or infectious disorders are disclosed, comprising obtaining a suitable sample from sources such as persons, animals, plants, food, water or soil. The methods also comprise providing tailored nucleic acid substrate(s) designed to react with a type 1 topoisomerase from one or more microorganism(s) or infectious agent(s), and incubating said substrate with said sample, or extracts or preparations from the sample, so that the substrate is processed by said topoisomerase if said microorganism(s) or infectious agent(s) is present in the sample. Microfluidic-implemented methods of detecting an enzyme, in particular a DNA-modifying enzyme, are also provided, as well as methods for detecting a cell, or a microorganism expressing said enzyme. The enzyme is detected by providing a nucleic acid substrate, which is specifically targeted by that enzyme.

System for identification of microorganism and detection of infectious disease

A scaffold assembly and related methods of manufacturing and/or using the scaffold for stem cell culture and tissue engineering applications are disclosed which at least partially mimic a native biological environment by providing biochemical, topographical, mechanical and electrical cues by using an electroactive material. The assembly includes at least one layer of substantially aligned, electrospun polymer fiber having an operative connection for individual voltage application. A method of cell tissue engineering and/or stem cell differentiation uses the assembly seeded with a sample of cells suspended in cell culture media, incubates and applies voltage to one or more layers, and thus produces cells and/or a tissue construct. In another aspect, the invention provides a method of manufacturing the assembly including the steps of providing a first pre-electroded substrate surface; electrospinning a first substantially aligned polymer fiber layer onto the first surface; providing a second pre-electroded substrate surface; electrospinning a second substantially aligned polymer fiber layer onto the second surface; and, retaining together the layered surfaces with a clamp and/or an adhesive compound.

Compositions and methods for modulating an immune response

Growing community of inventors

Durham, NC, United States of America

Kam W Leong