Handylab, Inc.

Bio-rad Laboratories, Inc.

Celsee Diagnostics, Inc.

Denovo Sciences, Inc.

University of Michigan

Becton Dickinson and Company Limited

Bio-rad Europe Gmbh

Venture Lending & Leasing Iv, Inc.

Denovo Sciecnes, Inc.

Denovo Science, Inc.

Venture Lending & Leasing V, Inc.

Kalyan Handique

Karthik Ganesan

Sundaresh N Brahmasandra

Priyadarshini Gogoi

Jeff Williams

Gene Parunak

Kyle Gleason

Austin Payne

Betty Wu

Christopher Siemer

Saedeh Sepehri Javdani

Aaron Kehrer

Kerry Wilson

Vishal Sharma

Yi Zhou

Nikhil Phadke

Brian Boniface

Saedeh Javdani Sepehri

John S Althaus

Cecelia Haley

Will Chow

Patrick Duffy

Ted Springer

Sam Tuck

Jon Meines

Daniel M Drummond

Mark A Burns

Ronald Lebofsky

John Stark

Yadwinder Deol

John Connolly

William Chow

Patrick Michael Tegels

Alec William Hitchiner

Katlyn Curtin Mehne

Grey Parker

Daniel Genord

William T Koederitz

Sida Wang

Thomas Catalino Gubatayao

Bishnu Prasanna Gogoi

Vijay Namasivayam

Swati Ranade

Lee Kyonghoon

Pridyadarshini Gogoi

William Weiyeh Chow

Carlos H Mastrangelo

John R Connolly

Josh Shinoff

Sophie Pierre

Christophe Quiring

Rebecca Dievart

Theodore Springer

Mark Travaglini

Eric Pomaranski

Emilie Celine Claudie Macke

Adam Mccoy

Deol Yadwinder

Will Koederitz

Priyadarshini Gogol

Inventions covered include methods, systems, and compositions for sample processing, involving morphology-adjustable (e.g., tunable on-demand) functionalized particles. In some embodiments, a method can include distributing a set of functionalized particles, in a first morphological state, across a set of partitions; transitioning the set of functionalized particles, at the set of partitions, from the first morphological state to a second morphological state; transitioning the set of functionalized particles, at the set of partitions, from the second morphological state to a third morphological state, and inducing interactions between the set of functionalized particles and a set of targets, within the set of partitions and according to a set of operations with a set of process fluids.

Compositions, methods, and systems for sample processing with morphology-adjustable functionalized particles

The present technology provides for a fluorescent detector that is configured to detect light emitted for a probe characteristic of a polynucleotide. The polynucleotide is undergoing amplification in a microfluidic channel with which the detector is in optical communication. The detector is configured to detect minute quantities of polynucleotide, such as would be contained in a microfluidic volume. The detector can also be multiplexed to permit multiple concurrent measurements on multiple polynucleotides concurrently.

Fluorescence detector for microfluidic diagnostic system

A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements; a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including mRNA capture, cDNA synthesis, protein-associated assays, and library preparation, for next generation sequencing.

System and method for automated single cell processing

The technology described herein generally relates to systems for extracting polynucleotides from multiple samples, particularly from biological samples, and additionally to systems that subsequently amplify and detect the extracted polynucleotides. The technology more particularly relates to microfluidic systems that carry out PCR on multiple samples of nucleotides of interest within microfluidic channels, and detect those nucleotides.

Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples

Methods and systems for sample target molecules are provided.

Apparatus and method for fast digital detection

A system for imaging captured cells comprising: an illumination module configured to illuminate a target object; a platform configured to position the target object in relation to the illumination module; a filter module configured to filter light transmitted to the target object and/or to filter light received from the target object, an optical sensor configured to receive light from the target object and to generate image data; and a focusing and optics module configured to manipulate light transmitted to the optical sensor. The system can further comprise one or more of: a control system configured to control at least one of the illumination module, the platform, the focusing and optics module, the filter module, and the optical sensor; a tag identifying system configured to identify and communicate tag information from system elements; a thermal control module configured to adjust temperature parameters of the system; and an image stabilization module.

System for imaging captured cells

This patent application describes an integrated apparatus for processing polynucleotide-containing samples, and for providing a diagnostic result thereon. The apparatus is configured to receive a microfluidic cartridge that contains reagents and a network for processing a sample. Also described are methods of using the apparatus.

Integrated system for processing microfluidic samples, and method of using same

A system, method, and platform for target detection, the system including: a base substrate; a set of sample processing regions defined at a broad surface of the substrate, wherein each of the set of sample processing regions includes: a set of microwell subarrays arranged in a gradient between an upstream end and a downstream end of each respective sample processing region, and a boundary separating each respective sample processing region from adjacent sample processing regions; and a cover substrate configured to mate with the base substrate in a coupled mode, the cover substrate comprising a network of venting channels aligned with the set of sample processing regions upon mating the base substrate with the cover substrate in the coupled mode, the network of venting channels providing gas exchange between the base substrate and an environment surrounding the microwell assembly. The invention(s) can be used for MPN assays.

System and method for target detection with applications in characterizing food quality and improving food safety

A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

System and method for retrieving and analyzing particles

A microfluidic device includes an input port for inputting a particle-containing liquidic samples into the device, a retention member, and a pressure actuator. The retention member is in communication with the input port and is configured to spatially separate particles of the particle-containing liquidic sample from a first portion of the liquid of the particle containing fluidic sample. The pressure actuator recombines at least some of the separated particles with a subset of the first portion of the liquid separated from the particles. The device can also include a lysing chamber that receives the particles and liquid from the retention member. The lysing chamber thermally lyses the particles to release contents thereof.

Processing particle-containing samples

The technology described herein generally relates to microfluidic cartridges configured to amplify and detect polynucleotides extracted from multiple biological samples in parallel. The technology includes a microfluidic substrate, comprising: a plurality of sample lanes, wherein each of the plurality of sample lanes comprises a microfluidic network having, in fluid communication with one another: an inlet; a first valve and a second valve; a first channel leading from the inlet, via the first valve, to a reaction chamber; and a second channel leading from the reaction chamber, via the second valve, to a vent.

Microfluidic cartridge

The present technology provides for a microfluidic substrate configured to carry out PCR on a number of polynucleotide-containing samples in parallel. The substrate can be a single-layer substrate in a microfluidic cartridge. Also provided are a method of making a microfluidic cartridge comprising such a substrate. Still further disclosed are a microfluidic valve suitable for use in isolating a PCR chamber in a microfluidic substrate, and a method of making such a valve.

Microfluidic valve and method of making same

The invention(s) cover systems and methods for target detection in a multiplexed and rapid manner. Embodiments of the system can include: a base substrate; and an array of sample processing regions defined at a broad surface of the base substrate, wherein each of the array of sample processing regions includes: a set of microwell subarrays arranged in a gradient by volumetric capacity between an upstream end and a downstream end of each respective sample processing region, and a boundary separating each respective sample processing region from adjacent sample processing regions. The system can support methods, with example implementation by an automated platform, for returning preliminary results from a subset of microwells of the samples processing regions, as well as results pertaining to specific and non-specific amplification, for multiple targets of a sample.

System and method for rapid multiplexed sample processing with applications for nucleic acid amplification assays

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Ypsilanti, MI, United States of America

Kalyan Handique