Richter Gedeon Vegyeszeti Gyar Rt

Biogal Gyogyszergyar

Kalman Konczol

Imre Moravcsik

Gabor Ambrus

Karoly Albrecht

Eva Ilkoy

Antonia Jekkel Nee Bokany

Gyula Horvath

Istvan M Szabo

Attila Andor

Tibor Lang

Janos Erdei

Valeria Szell

Antonia Jekkel

Istvan Pallagi

Gyorgy Santha

Ferenc Szederkenyi

Eva Toth-Sarudy

Emilia Simonovits

Kalman Polya

Bela Makadi

Nandor Makk

Eva Tomori Nee Joszt

Lajos Kiss

Karoly Nagy

Gabor Hantos

Vilma Szaszhegyesi

Zsuzsanna Lengyel Nee Szemenyei

Andras Toro

Eva Csajagi

Zsuzesanna Vida

Istvan Mihaly

Agota Hulber Nee Dobos

Bela Palotas

Karoly Buzasi

Etelka Deli Nee Konszky

Aniko Molnar Nee Antal

The invention relates to a novel process for the preparation of 17 .alpha.-hydroxy-20-oxopregnane derivatives of the general formula (I), ##STR1## wherein R.sub.1 means a hydroxy or an oxo group; and

Process for the preparation of steroids bearing

The invention relates to a microbial process for the production of immunosuppressive cyclosporine antibiotic complex or of its components, cyclosporine A, cyclosporine B and cyclosporine C, by the aerobic fermentation of a filamentous fungus strain biosynthesizing the above antibiotic(s) in a nutrient medium containing utilizable carbon and nitrogen sources as well as mineral salts, and by isolating the products formed, which comprises culturing a strain of the novel Tolypocladium varium fungus species producing the cyclosporine antibiotic complex, preferably tolypocladium varium sp. nov. CY/93, deposited at the National Collection of Agricultural and Industrial Microorganisms, Budapest, Hungary under the number NCAIM(P)F 001005, on a nutrient medium containing carbon sources, organic and inorganic nitrogen sources as well as mineral salts, under aerobic conditions, at 25.degree. to 30.degree. C., and, if desired, isolating and purifying the cyclosporine antibiotic complex or its components produced.

Microbial process for the production of immunosuppressive antibiotics

The invention relates to a process for the production of 9.alpha.-hydroxy-4-androstene-3,17-dione from natural sterols of plant or animal origin or from mixtures thereof by the submerged aerobic fermentation of an enzyme-deficient, sterol-de-grading microorganism in a nutrient medium containing utilizable carbon and nitrogen sources as well as mineral salts, and by isolating the product formed, which comprises transforming the sterol or sterol mixture with the culture of a 1,2-steroid-dehydrogenase deficient strain of new Mycobacterium roseum species which is able to degrade the side-chain of natural sterols, preferably with Mycobacterium roseum sp. nov. 1108/1 deposited at the National Collection of Agricultural and Industrial Microorganisms, Budapest, Hungary under the number NCAIM B (P) 000339, and by isolating and purifying the 9.alpha.-hydroxy-4-androstene-3,17-dione obtained.

Microbiological process for preparing

Growing community of inventors

Budapest, Hungary

Kalman Konczol